Metal enhanced fluorescence (MEF) for biosensors: General approaches and a review of recent developments
| Autor: | Yun Min Kook, Won Gun Koh, Yoon Jeong, Kangwon Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Fluorescence Electrochemistry Animals Humans Beneficial effects Nanoscopic scale Fluorescent Dyes Quenching (fluorescence) Equipment Design General Medicine Optical biosensor 021001 nanoscience & nanotechnology 0104 chemical sciences Spectrometry Fluorescence Metals 0210 nano-technology Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 111:102-116 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2018.04.007 |
| Popis: | Fluorescence-based biosensor platforms have been intensively investigated not only to increase the sensitivity but also to improve the performance of biosensors. By exploiting metal from the macroscopic down to the nanoscopic surface, various architectures have been devised to manipulate fluorescence signals (enhancement, quenching) within near-optical fields. The interaction of a metallic surface with proximal fluorophores (in the range of 5–90 nm) has beneficial effects on optical properties such as an increased quantum yield, improved photostability and a reduced lifetime of fluorophores. This phenomenon called metal-enhanced fluorescence (MEF) has been extensively used in biosensory applications. However, their applications for biological analysis practically remain challenging in biological microenvironments. Therefore, this review primarily provides a general overview of MEF biosensor systems from the basic mechanism to state-of-the-art biological applications. The review also covers the pros and cons of MEF biosensor as well as discussions about further directions in biological perspectives. |
| Databáze: | OpenAIRE |
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