Two splicing factors carrying serine-arginine motifs, TSR1 and TSR1IP, regulate splicing, mRNA stability, and rRNA processing inTrypanosoma brucei
| Autor: | Shulamit Michaeli, Ilana Naboishchikov, Hiba Waldman Ben-Asher, Sachin Kumar Gupta, Dror Eliaz, Itai Dov Tkacz, Vaibhav Chikne, Shai Carmi |
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| Rok vydání: | 2014 |
| Předmět: |
RNA
Spliced Leader Small RNA Transcription Genetic RNA Splicing RNA Stability Amino Acid Motifs Trypanosoma brucei brucei Protozoan Proteins RNA-binding protein Biology Mass Spectrometry Trans-Splicing Exon SR protein Gene Order Cluster Analysis Protein Interaction Domains and Motifs Gene Silencing RNA Messenger RRNA processing Molecular Biology Cell Nucleus Genetics AU-rich element Gene Expression Profiling RNA-Binding Proteins RNA RNA 3' Polyadenylation Signals Cell Biology Cell biology Protein Transport Genetic Loci RNA Ribosomal RNA splicing Transcriptome Research Paper |
| Zdroj: | RNA Biology. 11:715-731 |
| ISSN: | 1555-8584 1547-6286 |
| Popis: | In trypanosomes, mRNAs are processed by trans-splicing; in this process, a common exon, the spliced leader, is added to all mRNAs from a small RNA donor, the spliced leader RNA (SL RNA). However, little is known regarding how this process is regulated. In this study, we investigated the function of two serine-arginine-rich proteins, TSR1 and TSR1IP, implicated in trans-splicing in Trypanosoma brucei. Depletion of these factors by RNAi suggested their role in both cis- and trans-splicing. Microarray was used to examine the transcriptome of the silenced cells. The level of hundreds of mRNAs was changed, suggesting that these proteins have a role in regulating only a subset of T. brucei mRNAs. Mass-spectrometry analyses of complexes associated with these proteins suggest that these factors function in mRNA stability, translation, and rRNA processing. We further demonstrate changes in the stability of mRNA as a result of depletion of the two TSR proteins. In addition, rRNA defects were observed under the depletion of U2AF35, TSR1, and TSR1IP, but not SF1, suggesting involvement of SR proteins in rRNA processing. |
| Databáze: | OpenAIRE |
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