Comparison of the effects of two therapeutic strategies based on olfactory ensheathing cell transplantation and repetitive magnetic stimulation after spinal cord injury in female mice

Autor: Quentin Delarue, Romane Massardier, Amandine Robac, Jean-Paul Marie, Nicolas Guérout
Přispěvatelé: Groupe de Recherche sur le Handicap Ventilatoire (GRHV), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Delarue, Quentin
Rok vydání: 2021
Předmět:
RRID:AB_306827: p75
Abcam
ab8874
RRID:AB_839504: Iba1
Wako
019‐19741
0301 basic medicine
MRG2b-85
Cell Transplantation
712-165-153
RRID:AB_2340667: Jackson ImmunoResearch
Stimulation
RRID:AB_2313568: Jackson ImmunoResearch
RRID:AB_777165:P DGFRβAbcam ab32570
Pharmacology
RRID:AB_306827: p75
RRID:AB_94975: MBP
Millipore
MAB386
Mice
Random Allocation
RRID:AB_2340812: Jackson ImmunoResearch
0302 clinical medicine
Neural Stem Cells
Fibrosis
RRID:AB_476889: GFAP Cy3‐conjugated Sigma‐Aldrich
C9205
RRID:AB_2715913: Alexa 488
RRID:AB_2340667: Jackson ImmunoResearch
712‐165‐153
RRID:AB_10643424: PE
GFP)L2G85Chco/J)
Spinal cord injury
Research Articles
RRID:AB_10563302: PDGFRβ
MAB386
RRID:AB_2340812: Jackson ImmunoResearch
715‐165‐140
RRID:AB_10643424: PE
poly4064
BioLegend
406408
Wako
Olfactory Bulb
Transcranial Magnetic Stimulation
RRID:AB_476889: GFAP Cy3-conjugated Sigma-Aldrich
Microglial cell activation
Abcam
RRID:IMSR_JAX:008450: L2G85Chco+/+ (FVB-Tg(CAG-luc
[SDV.IMM]Life Sciences [q-bio]/Immunology
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Female
glial scar
RRID:IMSR_JAX:008450: L2G85Chco+/+ (FVB‐Tg(CAG‐luc
‐GFP)L2G85Chco/J)
Research Article
RRID:AB_2715913: Alexa 488
MRG2b‐85
BioLegend
C9205
RRID:AB_2313568: Jackson ImmunoResearch
711‐166‐152
[SDV.IMM] Life Sciences [q-bio]/Immunology
RRID:AB_10563302: PDGFRβ
Abcam
ab91066
711-166-152
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
rehabilitation
Glial scar
03 medical and health sciences
Cellular and Molecular Neuroscience
019-19741
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
poly4064
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
BioLegend
ab8874
RRID:AB_94975: MBP
magnetic stimulation
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
Spinal Cord Injuries
business.industry
RRID:AB_839504: Iba1
Recovery of Function
715-165-140
medicine.disease
ab91066
Neuroregeneration
spinal cord injury
Nerve Regeneration
Mice
Inbred C57BL
Transplantation
030104 developmental biology
Millipore
Olfactory ensheathing glia
business
olfactory ensheathing cells and neuroregeneration
030217 neurology & neurosurgery
Zdroj: Journal of Neuroscience Research
Journal of Neuroscience Research, 2021, 99 (7), pp.1835-1849. ⟨10.1002/jnr.24836⟩
ISSN: 1097-4547
0360-4012
DOI: 10.1002/jnr.24836
Popis: Spinal cord injury (SCI) is a debilitating condition, which leads to a permanent loss of functions below the injury site. The events which take place after SCI are characterized by cellular death, release of inhibitory factors, and inflammation. Many therapies have been studied to cure SCI, among them magnetic stimulation aims to reduce the secondary damages in particular by decreasing apoptosis, while, cellular transplantation promotes neuroregeneration by enhancing axonal regrowth. In the present study, we compared individually primary olfactory ensheathing cell (OEC) transplantation and repetitive trans‐spinal magnetic stimulation (rTSMS) and then, we combined these two therapeutic approaches on tissue repair and functional recovery after SCI. To do so, SCIs were performed at Th10 level on female C57BL/6 mice, which were randomized into four groups: SCI, SCI + primary bOECs, SCI + STM, SCI + primary bulbar olfactory ensheathing cells (bOECs) + stimulation (STM). On these animals bioluminescence, immunohistological, and behavioral experiments were performed after SCI. Our results show that rTSMS has beneficial effect on the modulation of spinal scar by reducing fibrosis, demyelination, and microglial cell activation and by increasing the astroglial component of the scar, while, primary bOEC transplantation decreases microglial reactivity. At the opposite, locotronic experiments show that both treatments induce functional recovery. We did not observed any additional effect by combining the two therapeutic approaches. Taken together, the present study indicates that primary bOEC transplantation and rTSMS treatment act through different mechanisms after SCI to induce functional recovery. In our experimental paradigm, the combination of the two therapies does not induce any additional benefit.
Summary of the main results of the study.
Databáze: OpenAIRE
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