High-affinity binding of a protein-lipopolysaccharide phytotoxin from Verticillium dahliae to cotton membranes
| Autor: | Ian A. Dubery, Riaan Meyer |
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| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
food.ingredient Biophysics Gossypium hirsutum Cotton Biology Biochemistry Binding site Fungal Proteins Phytotoxin food Structural Biology Genetics Verticillium dahliae Molecular Biology Gossypium Binding Sites Binding protein Cell Membrane Biological activity Cell Biology Hydrogen-Ion Concentration Mycotoxins Ligand (biochemistry) biology.organism_classification Kinetics Membrane Protein-lipopolysaccharide Mitosporic Fungi Cotyledon Protein Binding |
| Zdroj: | FEBS Letters. 335:203-206 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(93)80730-i |
| Popis: | The presence of a specific binding site for a protein-lipopolysaccharide (PLP) phytotoxin isolated from culture filtrates of Verticillium dahliae has been demonstrated in plasma membranes from cotton seedlings. The 125I-labelled PLP was used as a ligand in binding assays with plasma membrane enriched fractions. Root tissue exhibited the highest amount of binding activity compared to hypocotyl and cotyledon tissue. Binding of the [125I]PLP was saturable, reversible, and with an affinity (Kd = 1.42 × 10−8 M) comparable with the concentration required for biological activity. A single class of binding site was found, and the maximal number of binding sites were estimated as 5.4 × 10−15 mol μg protein. |
| Databáze: | OpenAIRE |
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