Xylem vessel‐specific SND5 and its homologs regulate secondary wall biosynthesis through activating secondary wall NAC binding elements
| Autor: | Chanhui Lee, Zheng-Hua Ye, Marziyeh Haghighat, Ruiqin Zhong |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Arabidopsis Plant Science 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Biosynthesis Cell Wall Gene Expression Regulation Plant Xylem Transcriptional regulation Arabidopsis thaliana Psychological repression Transcription factor Gene Plant Proteins biology Arabidopsis Proteins Chemistry biology.organism_classification Cell biology Populus 030104 developmental biology Secondary cell wall Transcription Factors 010606 plant biology & botany |
| Zdroj: | New Phytologist. 231:1496-1509 |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.17425 |
| Popis: | Secondary cell wall biosynthesis has been shown to be regulated by a suite of transcription factors. Here, we identified a new xylem vessel-specific NAC domain transcription factor, secondary wall-associated NAC domain protein5 (SND5), in Arabidopsis thaliana and studied its role in regulating secondary wall biosynthesis. We showed that the expression of SND5 and its close homolog, SND4/ANAC075, was specifically associated with secondary wall-containing cells and dominant repression of their functions severely reduced secondary wall thickening in these cells. Overexpression of SND4/5 as well as their homologs SND2/3 fused with the activation domain of the viral protein VP16 led to ectopic secondary wall deposition in cells that are normally parenchymatous. SND2/3/4/5 regulated the expression of the same downstream target genes as do the secondary wall NAC master switches (SWNs) by binding to and activating the secondary wall NAC binding elements (SNBEs). Furthermore, we demonstrated that the poplar (Populus trichocarpa) orthologs of SND2/3/4/5 also activated SNBEs and regulated secondary wall biosynthesis during wood formation. Together, these findings indicate that SND2/3/4/5 and their poplar orthologs regulate the expression of secondary wall-associated genes through activating SNBEs and they are positioned at an upper level in the SWN-mediated transcriptional network. |
| Databáze: | OpenAIRE |
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