An immunomagnetic separation and bifunctional Au nanoparticle probe-based multiamplification electrochemical strategy
| Autor: | Xiang Li, Chuan-Hua Zhou, Bing-Bing Shang, Xiao-Ru Zhang, Bao-Ping Qi, Wen‐sheng Huang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Working electrode Biophysics Nanoparticle Metal Nanoparticles 02 engineering and technology Biosensing Techniques Immunomagnetic separation 01 natural sciences chemistry.chemical_compound Limit of Detection Electrochemistry Humans Physical and Theoretical Chemistry Bifunctional Detection limit Immunoassay Reproducibility Chromatography 010401 analytical chemistry Reproducibility of Results General Medicine Electrochemical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Carcinoembryonic Antigen Transducer chemistry Magnets Gold 0210 nano-technology Selectivity Antibodies Immobilized |
| Zdroj: | Bioelectrochemistry (Amsterdam, Netherlands). 129 |
| ISSN: | 1878-562X |
| Popis: | A novel electrochemical magnetoimmunosensor for the rapid and sensitive detection of carcinoembryonic antigen (CEA) was fabricated based on a combination of high-efficiency immunomagnetic separation, bifunctional Au-nanoparticle (bi-AuNP) probes, and enzyme catalytic amplification. The reaction carrier magnetic beads (MBs) effectively reduced the toxicity of the complex sample to the working electrode, and the signal carrier bi-AuNP probes loaded a large amount of signal molecules, both of which enhanced the signal-to-noise ratio and further improved the detection sensitivity. A detection limit as low as 0.11 pg/mL was achieved for CEA detection based on the immunomagnetic separation and bi-AuNP probe-based multiamplification strategy, and the strategy was further successfully applied in human serum samples. The transducer was regenerated via a simple washing procedure, which enabled the detection of all samples on a single electrode with high reproducibility. The proposed strategy, which has the merits of high sensitivity, selectivity, and reproducibility exhibits great potential for detection in complex samples. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect