First time identification of Acanthamoeba genotypes in the cornea samples of wild birds; Is Acanthamoeba keratitis making the predatory birds a target?
| Autor: | Mert Döşkaya, Adnan Yüksel Gürüz, Ömer Döndüren, Esra Atalay Şahar, Hüseyin Can, Mehmet Aykur, Hande Dagci, Duygu Aldemir, Muhammet Karakavuk, Hüseyin Gökhan Özdemir, Mehmet Karakuş |
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| Přispěvatelé: | Uludağ Üniversitesi/Veteriner Fakültesi/İç Hastalıkları Anabilim Dalı., Aldemir, Duygu |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine Turkey RNA 18S Acanthamoeba Procedures Polymerase Chain Reaction Amebic keratitis Spp Wild birds Cornea 0302 clinical medicine Genotype Pathology Naegleria Fowleri Central Nervous System Protozoal Infections Eurasian sparrowhawk (Accipiter nisus) Canary-islands Ecology Acanthamoeba infection General Medicine Classification Chain-reaction analysis Molecular characterization Chemistry Veterinary Infectious Diseases medicine.anatomical_structure Protozoal DNA Peregrine falcon (Falco peregrinus) 030231 tropical medicine 030106 microbiology Immunology Sequencing data Animals Wild Biology Pathophysiology Keratitis Birds 03 medical and health sciences Bird parasitic diseases Genetics RNA Ribosomal 18S medicine Confocal microscopy diagnosis Animals Bird disease Animal Bird Diseases Wild animal Human health Water Accipiter DNA Protozoan medicine.disease biology.organism_classification eye diseases Isolation and purification Acanthamoeba Keratitis Acanthamoeba keratitis Noncontact lens wearers Pcr assay Parasitology |
| Zdroj: | Experimental Parasitology. 183:137-142 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2017.08.007 |
| Popis: | Acanthamoeba is a free-living amoeba which can be isolated from environment and among others well known as an opportunist protozoan parasite causing infections in humans and animals. Eyes are extremely important for the wild birds and losing sight ability due to Acanthamoeba can be dangerous. The studies on Acanthamoeba infection in wild birds is very few in world and Turkey therefore we aimed to screen deceased wild birds found in Izmir and Manisa provinces located in western Turkey using PCR and non-nutrition agar (NNA) plate method. Cornea samples were obtained from 18 deceased wild birds. During the external examination, signs of keratitis were observed in two Eurasian sparrowhawks (Accipiter nisus). All of the corneal samples were analyzed by two PCR methods and NNA plate. According to results, the Acanthamoeba positivity in corneal samples was 16.6% and 5.5% by PCR and plate method, respectively. According to sequencing data, two of isolates belonged to genotype T5 and one was genotype T4. In conclusion, Acanthamoeba infection was detected in wild bird cornea samples with/without keratitis for the first time in the world. The result of this study also show that Acanthamoeba can be a cause of keratitis in wild birds of Turkey and thus these predator birds can be a target of other wild animals due to loss of sight ability. In terms of public health, these results show the importance of wild birds as a source of Acanthamoeba infection in nature. Ege Üniversitesi - 2014-TIP-073 |
| Databáze: | OpenAIRE |
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