The Effect of Intra-articular versus Intravenous Injection of Mesenchymal Stem Cells on Experimentally-Induced Knee Joint Osteoarthritis
| Autor: | Ereny Fekry, Aya Mostafa, Horeya Erfan Korayem, Somaya Hosny |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Pathology medicine.medical_specialty H&E stain Osteoarthritis Knee Joint Stain Pathology and Forensic Medicine mono-iodoacetate 03 medical and health sciences 0302 clinical medicine medicine Animal model Instrumentation mesenchymal stem cells business.industry Mesenchymal stem cell medicine.disease Electronic Optical and Magnetic Materials Staining osteoarthritis 030104 developmental biology Nephrology 030220 oncology & carcinogenesis Original Article Stem cell business Perls' Prussian blue |
| Zdroj: | Journal of Microscopy and Ultrastructure |
| ISSN: | 2213-8803 |
| Popis: | Background Osteoarthritis (OA) is a chronic degenerative debilitating disease, primarily affects joints, particularly weight-bearing areas. The surface layer of the articular cartilage breaks down and wears away leading to rubbing of bones, pain, swelling, and joint stiffness. Aim and objectives This study investigates the possible therapeutic effects of intra-articular versus intravenous injection of umbilical cord blood mesenchymal stem cells (UCB-MSCs) against mono-iodoacetate-induced OA of the knee joints in male albino rats, using histological and immunohistochemical techniques. Materials and methods Thirty male adult albino rats were randomized into five groups as follows: Group (I) and (II): Served as control. Group (III): Osteoarthritic group. Group IV: Osteoarthritic and intraarticularly-injected MSCs. Group V: Osteoarthritic and intravenously-injected MSCs. Animals were sacrificed 1 month after stem cell injection, the right knee was prepared for histological techniques (Hematoxylin and Eosin and Toluidine blue stains) and immunohistochemical technique (Bax stain). Prussian blue stain was used to assess homing of MSCs in Groups IV and V. Results Knee joint surface was irregular, fissured, and fragmented in Group III. In Groups IV and V, affected area was filled with newly formed tissue. Toluidine blue showed a decrease in matrix staining in Group III compared to both control and MSCs-treated groups. Chondrocytes in Group III showed strong Bax immunoreactivity and this reaction decreased in Group IV and V; however, Group V immunoreactivity was more than Group IV. Prussian blue stain showed labeled UCB-MSCs in many chondrocytes in Group IV and few chondrocytes in Group V. Conclusion Intraarticularly-injected UCB-MSCs showed better healing of knee OA than intravenously-injected UCB-MSCs. |
| Databáze: | OpenAIRE |
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