Limiting rates of ligand association to alcohol dehydrogenase
| Autor: | David S. Sigman, Donald J. Creighton, Marianne Frolich |
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| Rok vydání: | 1978 |
| Předmět: |
Denticity
Macromolecular Substances Pyridines Biophysics Inner sphere electron transfer Photochemistry Biochemistry Dissociation (chemistry) 2 2'-Dipyridyl Chelation Molecular Biology Ternary complex biology Chemistry Ligand Water Active site NAD Alcohol Oxidoreductases Kinetics Zinc Benzophenoneidum Outer sphere electron transfer biology.protein Decanoic Acids Phenanthrolines Protein Binding |
| Zdroj: | Archives of Biochemistry and Biophysics. 189:471-480 |
| ISSN: | 0003-9861 |
| DOI: | 10.1016/0003-9861(78)90236-9 |
| Popis: | Detailed stopped-flow kinetic studies of the association of 2,2-bipyridine, 1,10-phenanthroline, and 5-chloro-1,10-phenanthroline to the zinc ion at the active site of alcohol dehydrogenase have demonstrated that a process with a limiting rate constant of about 200 s−1 restricts the binding of the bidentate chelating agents to the free enzyme. The formation of the enzyme-ligand complexes has been followed by means of the characteristic absorption spectra of the resulting complexes or by the displacement of the fluorescent dye, auramine O. Monodentate ligands, upon binding to the free enzyme or enzyme-NAD+ and enzyme-NADH complexes, do not exhibit a comparable limiting rate. In analogy with simple inorganic systems, these observations have been interpreted in terms of the rate limiting dissociation of an inner sphere water molecule following the rapid formation by the bidentate ligand of an outer sphere complex. The displacement of a water molecule from the zinc ion by 1,10-phenanthroline has been observed in crystallographic studies which have also established that the zinc ion in the enzyme-1,10-phenanthroline complex is pentacoordinate. Monodentate ligands, which are substrate analogs, do not exhibit limiting rates because displacement of water is not required for their addition to a coordinate position which is apparently vacant in the free enzyme. If a water molecule remains bound to the zinc ion in the kinetically competent ternary complex, it could play an essential role in the proton transfer reaction accompanying catalysis. |
| Databáze: | OpenAIRE |
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