Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis
| Autor: | Thomas Lecuit, Ankita Jha, Akankshi Munjal, Jean-Marc Philippe, Alain Garcia De Las Bayonas, Andrew J. Saurin, Stephen Kerridge |
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| Přispěvatelé: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Collège de France - Chaire Dynamiques du vivant, Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
rho GTP-Binding Proteins Myosin light-chain kinase G protein Morphogenesis macromolecular substances [SDV.BC]Life Sciences [q-bio]/Cellular Biology Epithelium 03 medical and health sciences 0302 clinical medicine Heterotrimeric G protein Myosin Animals Drosophila Proteins G protein-coupled receptor Myosin Type II biology Cell Polarity Apical constriction Epithelial Cells Cell Biology Actomyosin biology.organism_classification Actins Cell biology 030104 developmental biology Drosophila melanogaster Drosophila 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Nature Cell Biology Nature Cell Biology, Nature Publishing Group, 2016, 18 (3), pp.261-70. ⟨10.1038/ncb3302⟩ Nature Cell Biology, 2016, 18 (3), pp.261-70. ⟨10.1038/ncb3302⟩ |
| ISSN: | 1465-7392 1476-4679 |
| Popis: | International audience; Polarized cell shape changes during tissue morphogenesis arise by controlling the subcellular distribution of myosin II. For instance, during Drosophila melanogaster gastrulation, apical constriction and cell intercalation are mediated by medial-apical myosin II pulses that power deformations, and polarized accumulation of myosin II that stabilizes these deformations. It remains unclear how tissue-specific factors control different patterns of myosin II activation and the ratchet-like myosin II dynamics. Here we report the function of a common pathway comprising the heterotrimeric G proteins Gα12/13, Gβ13F and Gγ1 in activating and polarizing myosin II during Drosophila gastrulation. Gα12/13 and the Gβ13F/γ1 complex constitute distinct signalling modules, which regulate myosin II dynamics medial-apically and/or junctionally in a tissue-dependent manner. We identify a ubiquitously expressed GPCR called Smog required for cell intercalation and apical constriction. Smog functions with other GPCRs to quantitatively control G proteins, resulting in stepwise activation of myosin II and irreversible cell shape changes. We propose that GPCR and G proteins constitute a general pathway for controlling actomyosin contractility in epithelia and that the activity of this pathway is polarized by tissue-specific regulators. |
| Databáze: | OpenAIRE |
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