Potential therapeutic effect of curcumin loaded hyalurosomes against inflammatory and oxidative processes involved in the pathogenesis of rheumatoid arthritis: The use of fibroblast-like synovial cells cultured in synovial fluid
| Autor: | Xavier Fernàndez-Busquets, Anna Maria Maccioni, Maria Letizia Manca, Donatella Lattuada, Maria Manconi, Marco Zaru, Costantino Corradini, Ornella Marelli, Anna Maria Fadda, Donatella Valenti |
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| Rok vydání: | 2018 |
| Předmět: |
Curcumin
Sodium hyaluronate Pharmaceutical Science 02 engineering and technology Pharmacology medicine.disease_cause 030226 pharmacology & pharmacy Antioxidants Arthritis Rheumatoid 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Synovial Fluid medicine Synovial fluid Humans Hyaluronic Acid Cells Cultured Phospholipids Cell Proliferation chemistry.chemical_classification Reactive oxygen species Drug Carriers Vesicle Anti-Inflammatory Agents Non-Steroidal Synovial Membrane General Medicine Fibroblasts 021001 nanoscience & nanotechnology medicine.disease Oxidative Stress chemistry Synovial Cell Rheumatoid arthritis Inflammation Mediators 0210 nano-technology Oxidative stress Biotechnology |
| Zdroj: | European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V. 136 |
| ISSN: | 1873-3441 |
| Popis: | In the present work curcumin loaded hyalurosomes were proposed as innovative systems for the treatment of rheumatoid arthritis. Vesicles were prepared using a one-step and environmentally friendly method. Aiming at finding the most suitable formulation in terms of size, surface charge and stability on storage, an extensive pre-formulation study was performed using different type and amount of phospholipids. Curcumin loaded vesicles prepared with 180 mg/ml of Phospholipon 90G (P90G) and immobilized with sodium hyaluronate (2 mg/ml) were selected because of their small size (189 nm), homogeneous dispersion (PI 0.24), negative charge (−35 mV), suitable ability to incorporate high amount of curcumin (E% ∼88%) and great stability on storage. The in vitro study using fibroblast-like synovial cells cultured in synovial fluid, demonstrated the ability of these vesicles to downregulate the production of anti-apoptotic proteins IAP1 and IAP2 and stimulate the production of IL-10, while the production of IL-6 and IL-15 and reactive oxygen species was reduced, confirming their suitability in counteracting pathogenesis of rheumatoid arthritis. |
| Databáze: | OpenAIRE |
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