Aminopeptidase P and dipeptidyl peptidase IV activity in human leukocytes and in stimulated lymphocytes
| Autor: | M. van Sande, T. Umiel, G. Vanhoof, Arieh Yaron, D. Hendriks, I. De Meester, Simon Scharpé |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cell Extracts
Isoflurophate Dipeptidyl Peptidase 4 Lymphocyte Clinical Biochemistry Stimulation Biology Lymphocyte Activation Aminopeptidases Biochemistry Dipeptidyl peptidase Centrifugation Density Gradient Leukocytes medicine Humans Lymphocytes Phytohemagglutinins Dipeptidyl-Peptidases and Tripeptidyl-Peptidases Phytohaemagglutinin chemistry.chemical_classification Aminopeptidase P Biochemistry (medical) General Medicine medicine.anatomical_structure Enzyme chemistry biology.protein Specific activity Dipeptidyl peptidase IV activity |
| Zdroj: | Clinica chimica acta |
| ISSN: | 0009-8981 |
| Popis: | Human white blood cells were shown to contain high aminopeptidase P activity. The specific activities found in the high-speed supernatant of the extracts of granulocytes, lymphocytes and monocytes ranged from 30 to 70 units per mg protein. Culturing lymphocytes during 7 days in the presence of phytohaemagglutinin resulted in a 70–200% increase in the specific aminopeptidase P activity and a 200% increase in the specific activity of dipeptidyl peptidase IV. The time-course of the activity of both aminopeptidase P and dipeptidyl peptidase IV during the stimulation of human T-lymphocytes by phytohaemagglutinin indicates an involvement of these two enzymes in the proliferative process of these immunocompetent cells. Due to their substrate specificity their potential substrates must have the N-terminal Xaa-Pro sequence known to be present in several immunologically important polypeptides. |
| Databáze: | OpenAIRE |
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