Fabrication of antimicrobial silver-doped carbon structures by combinatorial pulsed laser deposition
| Autor: | Dorota Bociaga, Gianina Popescu-Pelin, Irina Zgura, Claudiu Hapenciuc, Ion N. Mihailescu, Coralia Bleotu, Irina Negut, Liviu Duta, Mariana Carmen Chifiriuc, Catalin Luculescu, Florin Miculescu, Cristina Besleaga, M.-A. Husanu, Gabriel Socol, George E. Stan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Silver
Materials science Biocompatibility Surface Properties Scanning electron microscope Pharmaceutical Science Nanotechnology Microbial Sensitivity Tests 02 engineering and technology Microscopy Atomic Force 010402 general chemistry 01 natural sciences Enterococcus faecalis Pulsed laser deposition symbols.namesake Anti-Infective Agents Coated Materials Biocompatible Staphylococcus epidermidis Bacteria biology Lasers Fungi Biofilm Spectrometry X-Ray Emission 021001 nanoscience & nanotechnology biology.organism_classification Carbon 0104 chemical sciences Carbon film Chemical engineering Biofilms Microscopy Electron Scanning symbols 0210 nano-technology Raman spectroscopy |
| Zdroj: | International Journal of Pharmaceutics. 515:592-606 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2016.10.041 |
| Popis: | We report on the selection by combinatorial pulsed laser deposition of Silver-doped Carbon structures with reliable physical-chemical characteristics and high efficiency against microbial biofilms. The investigation of the films was performed by scanning electron microscopy, high resolution atomic force microscopy, energy dispersive X-Ray Spectroscopy, X-ray diffraction, Raman spectroscopy, bonding strength “pull-out” tests, and surface energy measurements. In vitro biological assays were carried out using a large spectrum of bacterial and fungal strains, i.e., Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Enterococcus faecalis and Candida albicans. The biocompatibility of the films obtained was evaluated on MG63 mammalian cell cultures. The optimal combination with reasonable physical-chemical properties, efficient protection against microbial colonization and beneficial effects on human cells was found for Silver-doped Carbon films containing 2 to 7 at.% silver. These mixtures can be used to fabricate safe and efficient coatings of metallic implants, with the goal to decrease the risk of implant associated biofilm infections which are difficult to treat and often responsible for implant failure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect