Interactions between different media and follicle-stimulating hormone supplementation on in vitro culture of preantral follicles enclosed in ovarian tissue derived from collared peccaries (Pecari tajacuLinneaus, 1758)
| Autor: | Laritza Ferreira de Lima, Rmp Rocha, V. B. Luz, Alexandre Rodrigues Silva, José Ricardo de Figueiredo, S. V. Castro, A.P.R. Rodrigues, T.S. Castelo, Gabriela Liberalino Lima |
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| Rok vydání: | 2018 |
| Předmět: |
Tissue Culture Techniques
Andrology Extracellular matrix 03 medical and health sciences Follicle-stimulating hormone 0302 clinical medicine Endocrinology Ovarian Follicle Follicular phase Animals Artiodactyla 030219 obstetrics & reproductive medicine biology 0402 animal and dairy science Histology 04 agricultural and veterinary sciences 040201 dairy & animal science Staining Proliferating cell nuclear antigen biology.protein Immunohistochemistry Female Animal Science and Zoology Follicle Stimulating Hormone Biotechnology Hormone |
| Zdroj: | Reproduction in Domestic Animals. 53:880-888 |
| ISSN: | 0936-6768 |
| Popis: | The aim was to verify the effect of follicle-stimulating hormone (FSH) supplementation to α-MEM+ or TCM199+ media on the in vitro development of ovarian preantral follicles (PFs) derived from collared peccaries. Ovaries (n = 5 pairs) were collected and divided into fragments destined to control group (non-cultured) or treatments that were cultured for 7 days. The PFs morphology, growth and activation were evaluated by classical histology. The immunohistochemistry markers Ag-NOR and PCNA were used for nuclear proliferation analysis, and the picrosirius red labelling was used for ovarian extracellular matrix (ECM) evaluation. After 7-day culture, only the TCM199+ treatment maintained the proportion of intact PFs similar to day 1 (63.2%), but no differences were found among treatments (p > .05). In addition, a significant increase in the growing follicles proportion was verified for all the treatments, indicating follicular activation (p > .05). By the Ag-NOR analysis, only the TCM199+/FSH maintained the nuclear proliferation similar to the first day (p > .05). The picrosirius red staining revealed that the ECM remained intact in all the treatments (p > .05). We suggest the use of TCM199+ medium supplemented of FSH for the in vitro development of peccaries PFs under 7-day culturing conditions. |
| Databáze: | OpenAIRE |
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