Antitumor activity of spinasterol isolated from Pueraria roots
| Autor: | Myoung-Soon Park, Gook-Che Jeon, Chul-Ho Shin, Hong-Sig Sin, Soo-Jong Um, Do-Young Yoon |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Pueraria Clinical Biochemistry Stigmasterol Antineoplastic Agents Transfection Biochemistry High-performance liquid chromatography Plant Roots HeLa chemistry.chemical_compound Internal medicine medicine Tumor Cells Cultured Estrogen Receptor beta Humans Cytotoxicity Molecular Biology Chromatography High Pressure Liquid biology Traditional medicine Estrogen Receptor alpha biology.organism_classification Endocrinology Spinasterol chemistry Molecular Medicine Phytoestrogens Female Plant Preparations Estrogen receptor alpha Pueraria mirifica |
| Zdroj: | Experimentalmolecular medicine. 37(2) |
| ISSN: | 1226-3613 |
| Popis: | We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China. Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA- MB-231) in a dose- and time-dependent manner, as well as the growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17beta - estradiol, which activates both estrogen receptor alpha (ERalpha) and ERbeta. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3beta-ol) is an active cytotoxic component of Pueraria root. |
| Databáze: | OpenAIRE |
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