Safety and functional enrichment of gut microbiome in healthy subjects consuming a multi-strain fermented milk product: a randomised controlled trial
| Autor: | Laurent Quinquis, Philippe Marteau, Juergen Schrezenmeir, Muriel Derrien, Pierre Bourlioux, Julien Tap, Isabelle Chambaud, Anne-Sophie Alvarez, Eric Guillemard, Stéphanie Cools-Portier |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
DNA Bacterial Male Lactobacillus paracasei Cultured Milk Products lcsh:Medicine Gut flora Microbiology DNA Ribosomal Article law.invention Young Adult Probiotic Double-Blind Method Lactobacillus rhamnosus Randomized controlled trial law RNA Ribosomal 16S Clinical endpoint Humans Ingestion Food science lcsh:Science Phylogeny Multidisciplinary Ecology Bacteria biology Lacticaseibacillus rhamnosus Vital Signs Probiotics lcsh:R Gastroenterology food and beverages Sequence Analysis DNA Middle Aged biology.organism_classification Healthy Volunteers Gastrointestinal Microbiome Lactobacillus lcsh:Q Female Thyroid function |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-72161-w |
| Popis: | Many clinical studies have evaluated the effect of probiotics, but only a few have assessed their dose effects on gut microbiota and host. We conducted a randomized, double-blind, controlled intervention clinical trial to assess the safety (primary endpoint) of and gut microbiota response (secondary endpoint) to the daily ingestion for 4 weeks of two doses (1 or 3 bottles/day) of a fermented milk product (Test) in 96 healthy adults. The Test product is a multi-strain fermented milk product, combining yogurt strains and probiotic candidate strains Lactobacillus paracasei subsp. paracasei CNCM I-1518 and CNCM I-3689 and Lactobacillus rhamnosus CNCM I-3690. We assessed the safety of the Test product on the following parameters: adverse events, vital signs, hematological and metabolic profile, hepatic, kidney or thyroid function, inflammatory markers, bowel habits and digestive symptoms. We explored the longitudinal gut microbiota response to product consumption and dose, by 16S rRNA gene sequencing and functional contribution by shotgun metagenomics. Safety results did not show any significant difference between the Test and Control products whatever the parameters assessed, at the two doses ingested daily over a 4-week-period. Probiotic candidate strains were detected only during consumption period, and at a significantly higher level for the three strains in subjects who consumed 3 products bottles/day. The global structure of the gut microbiota as assessed by alpha and beta-diversity, was not altered by consumption of the product for four weeks. A zero-inflated beta regression model with random effects (ZIBR) identified a few bacterial genera with differential responses to test product consumption dose compared to control. Shotgun metagenomics analysis revealed a functional contribution to the gut microbiome of probiotic candidates. |
| Databáze: | OpenAIRE |
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