Identification of Human T-lymphotropic Virus Type I (HTLV-I) Subtypes Using Restrited Fragment Length Polymorphism in a Cohort of Asymptomatic Carriers and Patients with HTLV-I-associated Myelopathy/tropical Spastic Paraparesis from São Paulo, Brazil
| Autor: | Cristhieni Rodrigues, Cláudia Biasutti, Paulo E. Marchiori, Rogério Zeigler, Maria Lúcia S. G. Jorge, Cristine D. Damas, Aluísio Augusto Cotrim Segurado |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 viruses lcsh:QR1-502 Human T-lymphotropic virus Polymerase Chain Reaction lcsh:Microbiology Cohort Studies subtype immune system diseases restricted fragment length polymorphism Genotype Tropical spastic paraparesis medicine Humans Aged Human T-lymphotropic virus 1 biology Molecular epidemiology virus diseases Middle Aged biology.organism_classification medicine.disease HTLV-I Virology Paraparesis Tropical Spastic São Paulo Carrier State DNA Viral Htlv i associated myelopathy Female Restriction fragment length polymorphism Nested polymerase chain reaction Asymptomatic carrier Polymorphism Restriction Fragment Length Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 97, Iss 3, Pp 329-333 (2002) Memórias do Instituto Oswaldo Cruz, Volume: 97, Issue: 3, Pages: 329-333, Published: APR 2002 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | Although human T-lymphotropic virus type I (HTLV-I) exhibits high genetic stability, as compared to other RNA viruses and particularly to human immunodeficiency virus (HIV), genotypic subtypes of this human retrovirus have been characterized in isolates from diverse geographical areas. These are currently believed not to be associated with different pathogenetic outcomes of infection. The present study aimed at characterizing genotypic subtypes of viral isolates from 70 HTLV-I-infected individuals from São Paulo, Brazil, including 42 asymptomatic carriers and 28 patients with HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), using restricted fragment length polymorphism (RFLP) analysis of long terminal repeat (LTR) HTLV-I proviral DNA sequences. Peripheral blood mononuclear cell lysates were amplified by nested polymerase chain reaction (PCR) and amplicons submitted to enzymatic digestion using a panel of endonucleases. Among HTLV-I asymptomatic carriers, viral cosmopolitan subtypes A, B, C and E were identified in 73.8%, 7.1%, 7.1% and 12% of tested samples, respectively, whereas among HAM/TSP patients, cosmopolitan A (89.3%), cosmopolitan C (7.1%) and cosmopolitan E (3.6%) subtypes were detected. HTLV-I subtypes were not statistically significant associated with patients' clinical status. We also conclude that RFLP analysis is a suitable tool for descriptive studies on the molecular epidemiology of HTLV-I infections in our environment. |
| Databáze: | OpenAIRE |
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