Genetic and pathogenic characterization of a novel recombinant avian infectious bronchitis virus derived from GI-1, GI-13, GI-28, and GI-19 strains in Southwestern China
| Autor: | Xin Yang, Hao Li, Yaru Zhai, Pengfei Cui, Chang-Wei Lei, Lan Zhang, Rongbin Qiu, Kui Gu, Hongning Wang, Fuyan Wang, Xue Fu, Chun Li, Lei Zuo, Wenjun Yan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serotype
infectious bronchitis virus China Infectious bronchitis virus Biology Recombinant virus Genetic recombination SF1-1100 03 medical and health sciences Animals pathogenicity Phylogeny Poultry Diseases 030304 developmental biology GENETICS AND MOLECULAR BIOLOGY 0303 health sciences Attenuated vaccine 0402 animal and dairy science Outbreak 04 agricultural and veterinary sciences General Medicine Avian infectious bronchitis biology.organism_classification 040201 dairy & animal science Virology recombination Animal culture Animal Science and Zoology Avian infectious bronchitis virus Coronavirus Infections Chickens |
| Zdroj: | Poultry Science, Vol 100, Iss 7, Pp 101210-(2021) Poultry Science |
| ISSN: | 0032-5791 |
| Popis: | Avian infectious bronchitis (IB), caused by avian infectious bronchitis virus (IBV), is an acute and highly contagious disease that is extremely harmful to the poultry industry throughout the world. The cross-using of different attenuated live vaccine strains has led to the occurrence of diverse IBV serotypes. In this study, we isolated an IBV strain from a chicken farm in southwest China and designated it CK/CH/SCMY/160315. Construction of a phylogenetic tree based on full S1 gene sequence analysis suggested that CK/CH/SCMY/160315 bears similarity to GI-28, and further comparison of S1 amino acid residues revealed that CK/CH/SCMY/160315 showed mutations and deletions in many key positions between LDT3-A and other GI-28 reference strains. Importantly, CK/CH/SCMY/160315 was identified as a novel recombinant virus derived from live attenuated vaccine strains H120 (GI-1), 4/91 (GI-13), LDT3-A (GI-28), and the field strain LJL/08-1 (GI-19), identifying at least 5 recombination sites in both structural and accessory genes. Pathogenicity analysis indicated that CK/CH/SCMY/160315 caused listlessness, sneezing, huddling, head shaking, and increased antibody levels in the inoculated chickens. To further describe pathogenicity of this novel strain, we assessed viral load in different tissues and conducted hematoxylin and eosin (HE) staining of the trachea, lungs and kidneys. Our results provide evidence for the continuing evolution of IBV field strains via genetic recombination and mutation, leading to outbreaks in the vaccinated chicken populations in China. |
| Databáze: | OpenAIRE |
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