Schwann cells cultured from adult rats contain a cytoskeletal protein related to astrocyte filaments
| Autor: | Kay L. Fields, Paul McMenamin |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Fluorescent Antibody Technique
Schwann cell macromolecular substances Biology Protein filament Epitopes Developmental Neuroscience Antigen Glial Fibrillary Acidic Protein medicine Animals Intermediate Filament Protein Cytoskeleton Intermediate filament Cells Cultured Glial fibrillary acidic protein Molecular biology Rats Cell biology Cytoskeletal Proteins medicine.anatomical_structure Antigens Surface biology.protein Schwann Cells Developmental Biology Astrocyte |
| Zdroj: | Developmental Brain Research. 20:259-269 |
| ISSN: | 0165-3806 |
| DOI: | 10.1016/0165-3806(85)90113-0 |
| Popis: | Cells that bound antibody to the astrocyte intermediate filament protein were cultured from adult rat sciatic nerve. The antigen was intracellular, finely filamentous, and formed perinuclear caps in response to colchicine, all properties of intermediate filaments. Cytoskeletal proteins of these cultures were separated by SDS-gel electrophoresis, transferred to nitrocellulose paper, and shown to bind the glial-specific antiserum to a protein of 50,000 daltons. All the cells that bound this serum had a Schwann cell surface antigen, Ran-1, whereas fibroblasts from the nerve had Thy-1 surface antigen and did not contain the astrocyte filament antigen. These results prove that some Schwann cells from adult nerve, in contrast to fibroblasts or immature Schwann cells, have an intermediate filament protein that shares antigenic determinants with, or may be identical to, the astrocyte filament protein. |
| Databáze: | OpenAIRE |
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