Constitutive PI3-K activity is essential for proliferation, but not survival, of Theileria parva-transformed B cells
| Autor: | William C. Davis, Marie Chaussepied, Marie-Françoise Moreau, Dirk Werling, Martin Baumgartner, Gordon Langsley, Alphonse Garcia |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Transcriptional Activation
Cell Survival Theileria parva Immunology Lymphoproliferative disorders Microbiology Phosphatidylinositol 3-Kinases Virology Theileria medicine Animals Autocrine signalling Cell Line Transformed B-Lymphocytes biology Intracellular parasite NF-kappa B Granulocyte-Macrophage Colony-Stimulating Factor biology.organism_classification medicine.disease Colony-stimulating factor Phenotype Cell biology Transcription Factor AP-1 Cell culture Cattle Cell Division |
| Zdroj: | Cellular Microbiology. 2:329-339 |
| ISSN: | 1462-5822 1462-5814 |
| Popis: | Theileria is an intracellular parasite that causes lymphoproliferative disorders in cattle, and infection of leucocytes induces a transformed phenotype similar to tumour cells, but the mechanisms by which the parasite induces this phenotype are not understood. Here, we show that infected B lymphocytes display constitutive phosphoinositide 3-kinase (PI3-K) activity, which appears to be necessary for proliferation, but not survival. Importantly, we demonstrate that one mechanism by which PI3-K mediates the proliferation of infected B lymphocytes is through the induction of a granulocyte-monocyte colony-stimulating factor (GM-CSF)-dependent autocrine loop. PI3-K induction of GM-CSF appears to be at the transcriptional level and, consistently, we demonstrate that PI3-K is also involved in the constitutive induction of AP-1 and NF-kappaB, which characterizes Theileria-infected leucocytes. Taken together, our results highlight a novel strategy exploited by the intracellular parasite Theileria to induce continued proliferation of its host leucocyte. |
| Databáze: | OpenAIRE |
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