Postmortem diagnosis of autochthonous acute chagasic myocarditis by polymerase chain reaction amplification of a species-specific DNA sequence of Trypanosoma cruzi
| Autor: | Diane E. Ochs, David R. Moser, Jerome H. Smith, Violette S. Hnilica, Louis V. Kirchhoff |
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| Rok vydání: | 1996 |
| Předmět: |
Chagas disease
Chagas Cardiomyopathy Male Pathology medicine.medical_specialty Myocarditis Trypanosoma cruzi Molecular Sequence Data Autopsy Biology Polymerase Chain Reaction law.invention Fatal Outcome law Virology medicine Animals Humans Polymerase chain reaction Cause of death Postmortem Diagnosis Base Sequence Infant Sequence Analysis DNA DNA Protozoan medicine.disease biology.organism_classification Infectious Diseases Acute Disease Parasitology Trypanosomiasis |
| Zdroj: | The American journal of tropical medicine and hygiene. 54(5) |
| ISSN: | 0002-9637 |
| Popis: | We report a fatal case of vector-transmitted acute Chagas' myocarditis in a seven-month-old child in south Texas. This diagnosis was not suspected during the three days of hospitalization that preceded the child's death, which was caused by heart failure. A diagnosis of acute myocarditis, probably of viral origin, was listed as the cause of death after cardiac tissue was examined microscopically at autopsy. One year after the death of the patient, a diagnosis of Trypanosoma cruzi myocarditis, based solely on morphological grounds, was made after newly prepared slides of cardiac tissue were examined. Seven years later, we confirmed the diagnosis of T. cruzi infection by using the polymerase chain reaction to amplify a species-specific genomic repetitive DNA sequence of the parasite from fixed cardiac tissue. |
| Databáze: | OpenAIRE |
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