Enhanced production of styrene by engineered Escherichia coli and in situ product recovery (ISPR) with an organic solvent

Autor:	Hyun Bae Bang, Kyungsoo Lee, Ki Jun Jeong, Yoon Hyeok Lee
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	lcsh:QR1-502 Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology In situ product recovery (ISPR) lcsh:Microbiology Styrene chemistry.chemical_compound Bioreactors medicine Bioreactor Escherichia coli Food science Fed-batch culture chemistry.chemical_classification Research Polymer De novo synthesis Petrochemical Metabolic Engineering chemistry Batch Cell Culture Techniques Microorganisms Genetically-Modified Functional polymers Biotechnology
Zdroj:	Microbial Cell Factories, Vol 18, Iss 1, Pp 1-9 (2019) Microbial Cell Factories
ISSN:	1475-2859
Popis:	Background Styrene is a large-volume commodity petrochemical, which has been used in a wide range of polymer industry as the main building block for the construction of various functional polymers. Despite many efforts to produce styrene in microbial hosts, the production titers are still low and are not enough to meet the commercial production of styrene. Results Previously, we developed a high l-phenylalanine producer (E. coli YHP05), and it was used as a main host for de novo synthesis of styrene. First, we introduced the co-expression system of phenylalanine-ammonia lyase (PAL) and ferulic acid decarboxylase (FDC) genes for the synthesis of styrene from l-phenylalanine. Then, to minimize cell toxicity and enhance the recovery of styrene, in situ product recovery (ISPR) with n-dodecane was employed, and culture medium with supplementation of complex sources was also optimized. As a result, 1.7 ± 0.1 g/L of styrene was produced in the flask cultures. Finally, fed-batch cultivations were performed in lab-scale bioreactor, and to minimize the loss of volatile styrene during the cultivation, three consecutive bottles containing n-dodecane were connected to the air outlet of bioreactor for gas-stripping. To conclude, the total titer of styrene was as high as 5.3 ± 0.2 g/L, which could be obtained at 60 h. Conclusion We successfully engineered E. coli strain for the de novo production of styrene in both flask and fed-batch cultivation, and could achieve the highest titer for styrene in bacterial hosts reported till date. We believe that our efforts in strain engineering and ISPR strategy with organic solvent will provide a new insight for economic and industrial production of styrene in a biological platform. Electronic supplementary material The online version of this article (10.1186/s12934-019-1129-6) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
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