Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants
| Autor: | Audrey R. Odom John, Lucy B. Bollinger, Mwawi Mwale, Rachel Mlotha-Mitole, Indi Trehan, Barani Raman, Nalin Katta, Chad L Schaber |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Malawi Breath composition Plasmodium falciparum 030231 tropical medicine Gas Chromatography-Mass Spectrometry 03 medical and health sciences 0302 clinical medicine Anopheles parasitic diseases Animals Humans Immunology and Allergy Medicine Malaria Falciparum Child Malarial infection Volatile Organic Compounds biology business.industry Diagnostic test biology.organism_classification medicine.disease 030104 developmental biology Infectious Diseases Breath Tests Breath gas analysis Child Preschool Odorants Immunology Female business Biomarkers Malaria Malaria falciparum |
| Zdroj: | The Journal of Infectious Diseases. 217:1553-1560 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Current evidence suggests that malarial infection could alter metabolites in the breath of patients, a phenomenon that could be exploited to create a breath-based diagnostic test. However, no study has explored this in a clinical setting. To investigate whether natural human malarial infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from children with and those without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we found that children with malaria have a distinct shift in overall breath composition. Highly accurate classification of infection status was achieved with a suite of 6 compounds. In addition, we found that infection correlates with significantly higher breath levels of 2 mosquito-attractant terpenes, α-pinene and 3-carene. These findings attest to the viability of breath analysis for malaria diagnosis, identify candidate biomarkers, and identify plausible chemical mediators for increased mosquito attraction to patients infected with malaria parasites. |
| Databáze: | OpenAIRE |
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