Purification and characterization of rhodobactin: a mixed ligand siderophore from Rhodococcus rhodochrous strain OFS
| Autor: | Joseph G. Lack, Larry E. Hersman, Andrew T. Koppisch, Mitchell T. Johnson, Seth G. John, Jason M. Fairlee, Jennifer H. Forsythe, Matthew M. Cox, Ryszard Michalczyk, Laura A. Vanderberg, Hakim Boukhalfa, Cindy C. Browder, Mary P. Neu, Suraj Dhungana, Christy E. Ruggiero |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Siderophore Magnetic Resonance Spectroscopy Time Factors Epinephrine Stereochemistry Iron Siderophores Ligands Mass Spectrometry General Biochemistry Genetics and Molecular Biology Biomaterials Hydrolysis Bioremediation Rhodococcus Organic chemistry Chromatography High Pressure Liquid Strain (chemistry) biology Chemistry Metals and Alloys Rhodococcus rhodochrous Nuclear magnetic resonance spectroscopy Hydrogen-Ion Concentration biology.organism_classification Agar Models Chemical Metals Spectrophotometry Spectrophotometry Ultraviolet Peptides General Agricultural and Biological Sciences Two-dimensional nuclear magnetic resonance spectroscopy |
| Zdroj: | BioMetals. 20:853-867 |
| ISSN: | 1572-8773 0966-0844 |
| DOI: | 10.1007/s10534-006-9079-y |
| Popis: | The siderophore produced by Rhodococcus rhodochrous strain OFS, rhodobactin, was isolated from iron-deficient cultures and purified by a combination of XAD-7 absorptive/partition resin column and semi-preparative HPLC. The siderophore structure was characterized using 1D and 2D (1)H, (13)C and (15)N NMR techniques (DQFCOSY, TOCSY, NOESY, HSQC and LR-HSQC) and was confirmed using ESI-MS and MS/MS experiments. The structural characterization revealed that the siderophore, rhodobactin, is a mixed ligand hexadentate siderophore with two catecholate and one hydroxamate moieties for iron chelation. We further investigated the effects of Fe concentrations on siderophore production and found that Fe limiting conditions (Fe concentrations from 0.1 microM to 2.0 microM) facilitated siderophore excretion. Our interests lie in the role that siderophores may have in binding metals at mixed contamination sites (containing metals/radionuclides and organics). Given the broad metabolic capacity of this microbe and its Fe scavenging ability, R. rhodochrous OFS may have a competitive advantage over other organisms employed in bioremediation. |
| Databáze: | OpenAIRE |
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