Morphological dependent effect of cell-free formed supramolecular fibronectin on cellular activities
| Autor: | Hoang-Nghi Mai-Thi, Ngoc Quyen Tran, Volker R. Stoldt, Khon Huynh, Phong Minh Le |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Morphology (linguistics) Macromolecular Substances Clinical Biochemistry Fibril Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Cell Movement medicine Cell Adhesion Animals Urea Fibroblast Molecular Biology Cells Cultured Cell Proliferation biology Chemistry Fibrillogenesis Cell migration Adhesion Ligand (biochemistry) Fibronectins Fibronectin 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Biophysics |
| Zdroj: | Biological chemistryReferences. 402(2) |
| ISSN: | 1437-4315 |
| Popis: | Fibrillar fibronectin (FFN), an active form of fibronectin (FN), plays important roles in various cellular processes. Our goal is to investigate effect of FFN morphology on cellular behaviors. Plasma FN at two concentrations was cross-linked into FFN by dialysis against 2 M urea followed by morphological analysis under Scanning Electron Microscopy. To evaluate effect of FFN morphology, fibroblasts were cultured on FN or different FFNs. Fibroblast behaviors including adhesion, spreading, and migration were evaluated. Our data showed that FN fibrillogenesis was dependent on FN concentration. At high concentrations (0.75 mg/mL), large FFN approximately 2.167 + 0.875 µm in diameter were formed with attached nodular structures and rough surface. In contrast, smooth surface FFN fibrils with diameter of 1.886 + 0.412 µm were formed from FN at 0.25 mg/mL. Cellular assays revealed morphological dependent biological effects of different FFNs. Fibroblast separately adhered to native FN and remained spherical while on FFN, cells attached with higher quantity and showed spreading morphology. A synergistic ligand interaction of integrin α5β1 and αvβ3 was observed in cell adhering on FFN. Cell migration results showed that large FFN decreased migration rate while small FFN did not. Taken together, our data draws new attention towards controlling biological function of FN by its fibrillar structure. |
| Databáze: | OpenAIRE |
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