Oral contraceptive use induces upregulation of the CCR5 chemokine receptor on CD4+ T cells in the cervical epithelium of healthy women
| Autor: | Manyu Prakash, Frances Gotch, Moses S. Kapembwa, Steven Patterson |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Receptors CXCR4 medicine.medical_specialty Chemokine Receptors CCR5 T-Lymphocytes Immunology Population Cervix Uteri Biology Lymphocyte Activation CXCR4 Chemokine receptor Immune system Internal medicine medicine Humans Immunology and Allergy IL-2 receptor education education.field_of_study Obstetrics and Gynecology T lymphocyte Flow Cytometry Up-Regulation Endocrinology Reproductive Medicine biology.protein Female CD8 Contraceptives Oral |
| Zdroj: | Journal of Reproductive Immunology. 54:117-131 |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/s0165-0378(01)00125-5 |
| Popis: | Heterosexual transmission of human immunodeficiency virus type I (HIV-1) is the predominant mode of infection worldwide. Increased risk of HIV-1 transmission has been reported with oral contraceptive use. To elucidate the underlying mechanism of this observation, intraepithelial endocervical T lymphocytes from women using oral contraceptives were analysed for expression of activation and chemokine receptors. T lymphocytes from the cervical epithelium and peripheral blood of women using combined oral contraceptives (COC) and those using no contraceptive method (NONE) were compared. Cervical T lymphocytes were obtained with a cytobrush and in parallel, mononuclear leukocytes were separated from blood by centrifugation over a ficoll-hypaque gradient. Cellular activation markers and HIV-1 chemokine co-receptors, CXCR4 and CCR5, were analysed by flow cytometry. Activation markers (CD69, CD25 and HLA-DR) on T cells were expressed at higher levels in the cervical epithelium than peripheral blood T cells but were no different in those women using COC. CXCR4 was widely expressed on cervical and on blood T cells, but was not influenced by COC use. By contrast, the number of T cells expressing CCR5 increased in women using COC (P0.05). The level of cervical CCR5 expression per cell was shown to increase on both activated CD4(+) (CD69(+), P0.05; HLA-DR(+), P0.01) and CD8(+) (CD69(+), P0.05; HLA-DR(+), P0.05) T lymphocytes compared with COC use. These data show that with COC use, the expression of CCR5 on CD4(+) T lymphocytes is increased. Furthermore, the cell surface density of CCR5 is increased on both CD4(+) and CD8(+) T lymphocyte subsets. These findings suggest a mechanism by which oral contraceptive use can increase the risk of HIV-1 transmission. |
| Databáze: | OpenAIRE |
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