Influence of flanking sequences on variability in expression levels of an introduced gene in transgenic tobacco plants
| Autor: | Jonathan D. G. Jones, Mitchell Favreau, John Bedbrook, Pamela Dunsmuir, Caroline Dean |
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| Rok vydání: | 1988 |
| Předmět: |
Regulation of gene expression
Genetics Agrobacterium Ribulose-Bisphosphate Carboxylase Genetic Vectors DNA Recombinant Regulatory Sequences Nucleic Acid Biology Blotting Northern biology.organism_classification Molecular biology Petunia Plants Toxic Transformation (genetics) Transformation Genetic Gene Expression Regulation Regulatory sequence Tobacco Gene expression RNA Messenger Northern blot Gene |
| Zdroj: | Nucleic Acids Research. 16:9267-9283 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The petunia rbcS gene SSU301 was introduced into tobacco using Agrobacterium tumefaciens-mediated transformation. The time at which rbcS expression was maximal after transfer of the tobacco plants to the greenhouse was determined. The expression level of the SSU301 gene varied up to 9 fold between individual tobacco plants which had been standardized physiologically as much as possible. The presence of adjacent pUC plasmid sequences did not affect the expression of the SSU301 gene. In an attempt to reduce the between-transformant variability in expression, the SSU301 gene was introduced into tobacco surrounded by 10kb of 5' and 13 kb of 3' DNA sequences which normally flank SSU301 in petunia. The longer flanking regions did not reduce the between-transformant variability of SSU301 gene expression. |
| Databáze: | OpenAIRE |
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