Transport and compartmentation of phosphite in higher plant cells - kinetic and 31P nuclear magnetic resonance studies
| Autor: | Pieter de Waard, Margret Köck, Cor Dijkema, Ralitza Danova‐Alt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Physiology
Biophysics suspension cells Plant Science Vacuole tomato deprivation chemistry.chemical_compound Nuclear magnetic resonance Nuclear magnetic resonance spectroscopy Metabolism Compartmentalization (fire protection) Phosphate Plant cell physiological-responses inhibition arabidopsis fungicide phosphonate Biofysica chemistry Biochemistry phytophthora-palmivora saccharomyces-cerevisiae Efflux starvation responses Intracellular |
| Zdroj: | Plant, Cell & Environment, 31(10), 1510-1521 Plant, Cell & Environment 31 (2008) 10 |
| ISSN: | 0140-7791 |
| DOI: | 10.1111/j.1365-3040.2008.01861.x |
| Popis: | Phosphite (Phi, H(2)PO(3)(-)), being the active part of several fungicides, has been shown to influence not only the fungal metabolism but also the development of phosphate-deficient plants. However, the mechanism of phosphite effects on plants is still widely unknown. In this paper we analysed uptake, subcellular distribution and metabolic effects of Phi in tobacco BY-2 cells using in vivo(31)P nuclear magnetic resonance ((31)P-NMR) spectroscopy. Based on the kinetic properties of the phosphate transport system of tobacco BY-2 cells, it was demonstrated that phosphite inhibited phosphate uptake in a competitive manner. To directly follow the fate of phosphate and phosphite in cytoplasmic and vacuolar pools of tobacco cells, we took advantage of the pH-sensitive chemical shift of the Phi anion. The NMR studies showed a distinct cytoplasmic accumulation of Phi in Pi-deprived cells, whereas Pi resupply resulted in a rapid efflux of Phi. Pi-preloaded cells shifted Phi directly into vacuoles. These studies allowed for the first time to follow Phi flux processes in an in vivo setting in plants. On the other hand, the external Pi nutrition status and the metabolic state of the cells had a strong influence on the intracellular compartmentalization of xenobiotic Phi. |
| Databáze: | OpenAIRE |
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