Bacteria control cell volume by coupling cell-surface expansion to dry-mass growth
| Autor: | Baptiste Cordier, Gizem Özbaykal, Enno R. Oldewurtel, Yuki Kitahara, Sven van Teeffelen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chemistry Turgor pressure Cell Biomass Cell cycle Coupling (electronics) 03 medical and health sciences 0302 clinical medicine Nutrient medicine.anatomical_structure Volume (thermodynamics) Biophysics medicine 030217 neurology & neurosurgery Intracellular 030304 developmental biology |
| DOI: | 10.1101/769786 |
| Popis: | Cells exhibit a high degree of intracellular crowding. To control the level of crowding during growth cells must increase their volumes in response to the accumulation of biomass. UsingEscherichia colias a model organism, we found that cells control cell volume indirectly, by increasing cell-surface area in proportion to biomass growth. Thus, dry-mass density, a readout of intracellular crowding, varies in proportion to the surface-to-volume ratio, both during the cell cycle and during perturbations such as nutrient shifts. On long time scales after shifts, initial dry-mass density is nearly restored by slow variations of the surface-to-mass ratio. Contrary to a long-standing paradigm, cell-envelope expansion is controlled independently of cell-wall synthesis but responds to the activity of cell-wall cleaving hydrolases. Finally, we observed rapid changes of Turgor pressure after nutrient shifts, which were likely responsible for initial changes of cell diameter and dry-mass-density. Together, our experiments reveal important regulatory relationships for cell volume and shape. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|