Allopregnanolone alters the luteinizing hormone, prolactin, and progesterone serum levels interfering with the regression and apoptosis in rat corpus luteum
| Autor: | R. F. Yunes, Juan C. Cavicchia, Z. Y. Sosa, Ciro Estrada Chavez, R. J. Cabrera, Mabel Fóscolo, Myriam Raquel Laconi |
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| Rok vydání: | 2012 |
| Předmět: |
endocrine system
medicine.medical_specialty Endocrinology Diabetes and Metabolism media_common.quotation_subject Clinical Biochemistry Apoptosis Cell Count Pregnanolone Biology Luteal phase Biochemistry Prolactin cell Rats Sprague-Dawley chemistry.chemical_compound Endocrinology Corpus Luteum Internal medicine medicine Animals Ovulation Progesterone media_common Estrous cycle Biochemistry (medical) Allopregnanolone General Medicine Luteinizing Hormone Prolactin Rats medicine.anatomical_structure chemistry Oocytes Female Luteinizing hormone Corpus luteum hormones hormone substitutes and hormone antagonists |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 44(8) |
| ISSN: | 1439-4286 |
| Popis: | Steroids synthesized in the central nervous system are termed "neurosteroids". They are synthesized and metabolized in several brain areas. The objective of this work was to determine if 1 intracerebroventricular allopregnanolone injection in rats can interfere in luteal regression in a close relationship with modifications in LH, progesterone, and prolactin serum concentrations. Allopregnanolone was injected during proestrus morning and the animals were sacrificed on oestrous morning. Ovulation test and histological analysis were performed in the oestrus morning with light and electron microscopy. Serum prolactin, LH, and progesterone levels were measured by radioimmunoassay. The allopregnanolone injection significantly decreased luteinizing hormone serum level and the number of oocytes on oestrus. Progesterone and prolactin serum levels were increased after this injection. The inhibition of apoptotic figures due to allopregnanolone administration was detected in the already formed corpora lutea belonging to the previous ovary cycle and it was significantly lower than in vehicle group (control). When the GABA(A) antagonist (bicuculline) was administered alone or previously to allopregnanolone, no effect on the ovulation rate was observed. No changes in the apoptotic cell numbers were observed with respect to those of vehicle group. These results show that the effect of centrally injected allopreganolone over reproductive function could be due to a centrally originated LH mediated effect over ovarian function that affects luteal regression, through the inhibition of apoptosis and stimulation of progesterone and prolactin release. |
| Databáze: | OpenAIRE |
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