Deciphering the Role of CD1e Protein in Mycobacterial Phosphatidyl- myo -inositol Mannosides (PIM) Processing for Presentation by CD1b to T Lymphocytes
| Autor: | Emilie Layre, Diane Cala-De Paepe, Luis F. Garcia-Alles, Lucia Mori, Germain Puzo, Gaëlle Giacometti, Daniel Hanau, Martine Gilleron, Gennaro De Libero |
|---|---|
| Přispěvatelé: | Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Experimental Immunology, University Hospital Basel [Basel], Biologie des Cellules Dendritiques Humaines, EFS-Cancéropôle du Grand Est-Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH: Antigens
CD1 MESH: Mycobacterium tuberculosis [SDV.BIO]Life Sciences [q-bio]/Biotechnology T-Lymphocytes [SDV]Life Sciences [q-bio] Antigen presentation CD1 Biology Major histocompatibility complex Biochemistry Cell Line Antigens CD1 03 medical and health sciences 0302 clinical medicine Antigen alpha-Mannosidase hemic and lymphatic diseases Humans MESH: Glycolipids [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology Pan-T antigens 030304 developmental biology 0303 health sciences Antigen Presentation Antigens Bacterial MESH: Humans Antigen processing technology industry and agriculture [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology Mycobacterium tuberculosis Lipids MESH: Cell Line MESH: alpha-Mannosidase carbohydrates (lipids) MESH: T-Lymphocytes Immunoediting MESH: Antigen Presentation biology.protein lipids (amino acids peptides and proteins) Glycolipids Plant lipid transfer proteins MESH: Antigens Bacterial 030215 immunology |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (37), pp.31494-31502. ⟨10.1074/jbc.M112.386300⟩ The Journal of biological chemistry |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.M112.386300⟩ |
| Popis: | International audience; Lipids are important antigens that induce T cell-mediated specific immune responses. They are presented to T lymphocytes by a specific class of MHC-I like proteins, termed CD1. The majority of the described CD1-presented mycobacterial antigens are presented by the CD1b isoform. We previously demonstrated that the stimulation of CD1b-restricted T cells by the hexamannosylated phosphatidyl-myo-inositol (PIM(6)), a family of mycobacterial antigens, requires a prior partial digestion of the antigen oligomannoside moiety by α-mannosidase and that CD1e is an accessory protein absolutely required for the generation of the lipid immunogenic form. Here, we show that CD1e behaves as a lipid transfer protein influencing lipid immunoediting and membrane transfer of PIM lipids. CD1e selectively assists the α-mannosidase-dependent digestion of PIM(6) species according to their degree of acylation. Moreover, CD1e transfers only diacylated PIM from donor to acceptor liposomes and also from membranes to CD1b. This study provides new insight into the molecular mechanisms by which CD1e contributes to lipid immunoediting and CD1-restricted presentation to T cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|