Crystallization and preliminary X-ray diffraction studies of bovine recombinant immune interferon
| Autor: | J.R. Rubin, L.E. Burton |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Materials science
Protein Conformation Stereochemistry Dimer Crystal structure law.invention Interferon-gamma chemistry.chemical_compound Tetragonal crystal system X-Ray Diffraction Structural Biology law Interferon medicine Animals Crystallization Molecular Biology Resolution (electron density) Recombinant Proteins Crystallography chemistry X-ray crystallography Cattle Orthorhombic crystal system medicine.drug |
| Zdroj: | Journal of Molecular Biology. 209:829-831 |
| ISSN: | 0022-2836 |
| Popis: | Reproducible conditions have been established for the crystallization of recombinant bovine immune interferon. Two cystalline forms of this protein were obtained. A tetragonal form, space group P422, with unit cell dimensions a = b = 59.0 A and c = 125.7 A and an orthorhombic form, space group P2(1)2(1)2(1), with unit cell dimensions a = 42.80 A, b = 79.90 A and c = 85.64 A were obtained under similar crystallization conditions. The orthorhombic form diffracts to 2.6 A resolution, contains a single interferon dimer in the asymmetric unit of structure and is suitable for X-ray diffraction analysis. |
| Databáze: | OpenAIRE |
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