Immortalization of Human Endothelial Cells by Origin-defective Simian Virus 40 DNA
| Autor: | Mitsuyoshi Ishida, Tadashi Hishida, Takeshi Kobayashi, Sadayo Nakajima-Iijima, Shinji Iijima, Hideki Watanabe |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Transfection
Biology Tissue plasminogen activator Virology Molecular biology Virus General Biochemistry Genetics and Molecular Biology Endothelial stem cell chemistry.chemical_compound Antigen chemistry Cell culture medicine General Agricultural and Biological Sciences Plasminogen activator DNA medicine.drug |
| Zdroj: | Agricultural and Biological Chemistry. 55:2847-2853 |
| ISSN: | 1881-1280 0002-1369 |
| DOI: | 10.1271/bbb1961.55.2847 |
| Popis: | Human endothelial cells isolated from an umbilical cord vein were transfected with origin-defective simian virus 40 (SV40) DNA. Among several of the SV40 transfected clones isolated, cell lines SV-2 and SV-3 showed a normal endothelial cell morphology and extended life span, and could survive almost 100 generations. Just before crisis, the morphology of SV-3 changed. SV-3T cell line was isolated from this SV-3 culture, which acquired an almost infinite life span, rapid growth rate and the ability to grow in soft agar. At the same time, the SV-3T cell line lost the factor VIII-related antigen and normal endothelial cell morphology, and showed an abnormal chromosome number. Further characterization showed the ability of SV-2 and SV-3T to produce increasing amounts of tissue plasminogen activator and a similar level of a plasminogen activator inhibitor compared with normal human endothelial cells. These results indicate that the SV-3T cell line was transformed and acquired an infinite life span while still r... |
| Databáze: | OpenAIRE |
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