The Arabidopsis dwarf mutant shi exhibits reduced gibberellin responses conferred by overexpression of a new putative zinc finger protein
| Autor: | Thomas Moritz, Eva Sundberg, Ingela Fridborg, Sandra Kuusk |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Transposable element
Transcription Genetic Mutant Molecular Sequence Data Arabidopsis Plant Science Polymerase Chain Reaction Feedback Open Reading Frames Caulimovirus Amino Acid Sequence RNA Messenger Cloning Molecular Promoter Regions Genetic Peptide sequence Gene Genetics Zinc finger biology Base Sequence Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction fungi Zinc Fingers Cell Biology biology.organism_classification Gibberellins Open reading frame Mutagenesis Insertional Phenotype DNA Transposable Elements Cauliflower mosaic virus Polymorphism Restriction Fragment Length Transcription Factors Research Article |
| Zdroj: | The Plant cell. 11(6) |
| ISSN: | 1040-4651 |
| Popis: | shi (for short internodes), a semidominant dwarfing mutation of Arabidopsis caused by a transposon insertion, confers a phenotype typical of mutants defective in the biosynthesis of gibberellin (GA). However, the application of GA does not correct the dwarf phenotype of shi plants, suggesting that shi is defective in the perception of or in the response to GA. In agreement with this observation, the level of active GAs was elevated in shi plants, which is the result expected when feedback control of GA biosynthesis is reduced. Cloning of the SHI gene revealed that in shi, the transposon is inserted into the untranslated leader so that a cauliflower mosaic virus 35S promoter in the transposon reads out toward the SHI open reading frame. This result, together with mRNA analysis, suggests that the phenotype of the shi mutant is a result of overexpression of the SHI open reading frame. The predicted amino acid sequence of SHI has acidic and glutamine-rich stretches and shows sequence similarity over a putative zinc finger region to three presumptive Arabidopsis proteins. This suggests that SHI may act as a negative regulator of GA responses through transcriptional control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|