| Autor: |
Marzia Rizzo, Natthapon Soisangwan, Samuel Vega-Estevez, Robert Jordan Price, Chloe Uyl, Elise Iracane, Matt Shaw, Jan Soetaert, Anna Selmecki, Alessia Buscaino |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
PLOS Genetics. 18:e1010576 |
| ISSN: |
1553-7404 |
| DOI: |
10.1371/journal.pgen.1010576 |
| Popis: |
A delicate balance between genome stability and instability ensures genome integrity while generating genetic diversity, a critical step for evolution. Indeed, while excessive genome instability is harmful, moderated genome instability can drive adaptation to novel environments by maximising genetic variation.Candida albicans, a human fungal pathogen that colonises different parts of the human body, adapts rapidly and frequently to different hostile host microenvironments. In this organism, the ability to generate large-scale genomic variation is a key adaptative mechanism triggering dangerous infections even in the presence of antifungal drugs. Understanding how fitter novel karyotypes are selected is key to determining howC.albicansand other microbial pathogens establish infections. Here, we identified the SUMO protease Ulp2 as a regulator ofC.albicansgenome integrity through genetic screening. Deletion ofULP2leads to increased genome instability, enhanced genome variation and reduced fitness in the absence of additional stress. The combined stress caused by the lack ofULP2and antifungal drug treatment leads to the selection of adaptive segmental aneuploidies that partially rescue the fitness defects ofulp2Δ/Δ cells. Short and long-read genomic sequencing demonstrates that these novel genotypes are selected via a two-step process leading to the formation of novel chromosomal fragments with breakpoints at microhomology regions and DNA repeats. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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