A Selaginella lepidophylla Trehalose-6-Phosphate Synthase Complements Growth and Stress-Tolerance Defects in a Yeast tps1 Mutant1
| Autor: | Johan M. Thevelein, Beatriz M. Bonini, Jorge Nieto-Sotelo, Patrick Van Dijck, Gabriel Iturriaga, Roberto Gaxiola, Jorge Luis Folch-Mallol, José Oscar Mascorro-Gallardo, Rodolfo Zentella, Christophe Van Vaeck, Alejandra A. Covarrubias |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
DNA
Complementary Hot Temperature DNA Plant Physiology Saccharomyces cerevisiae ved/biology.organism_classification_rank.species Mutant Genes Fungal Molecular Sequence Data Gene Expression Resurrection plant Plant Science Genes Plant chemistry.chemical_compound Gene expression Selaginella lepidophylla Genetics Amino Acid Sequence Cloning Molecular DNA Primers biology Base Sequence Sequence Homology Amino Acid ved/biology Genetic Complementation Test Trehalose Plants biology.organism_classification Complementation Biochemistry chemistry Glucosyltransferases Mutation Phosphatase complex Research Article |
| Popis: | The accumulation of the disaccharide trehalose in anhydrobiotic organisms allows them to survive severe environmental stress. A plant cDNA,SlTPS1, encoding a 109-kD protein, was isolated from the resurrection plant Selaginella lepidophylla, which accumulates high levels of trehalose. Protein-sequence comparison showed that SlTPS1 shares high similarity to trehalose-6-phosphate synthase genes from prokaryotes and eukaryotes. SlTPS1mRNA was constitutively expressed in S. lepidophylla. DNA gel-blot analysis indicated that SlTPS1 is present as a single-copy gene. Transformation of a Saccharomyces cerevisiae tps1Δ mutant disrupted in theScTPS1 gene with S. lepidophylla SlTPS1restored growth on fermentable sugars and the synthesis of trehalose at high levels. Moreover, the SlTPS1 gene introduced into the tps1Δ mutant was able to complement both deficiencies: sensitivity to sublethal heat treatment at 39°C and induced thermotolerance at 50°C. The osmosensitive phenotype of the yeast tps1Δ mutant grown in NaCl and sorbitol was also restored by the SlTPS1 gene. Thus, SlTPS1 protein is a functional plant homolog capable of sustaining trehalose biosynthesis and could play a major role in stress tolerance in S. lepidophylla. |
| Databáze: | OpenAIRE |
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