A Protein Complex Containing Mei5 and Sae3 Promotes the Assembly of the Meiosis-Specific RecA Homolog Dmc1
| Autor: | Akira Shinohara, Hiroyuki Oshiumi, Misato Takagi, Toshiko Miyazaki, Miki Shinohara, Atsuko Hayase |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Saccharomyces cerevisiae Proteins
Chromosomal Proteins Non-Histone Molecular Sequence Data genetic processes RAD51 Cell Cycle Proteins Saccharomyces cerevisiae Biology Genetic recombination Chromosomes General Biochemistry Genetics and Molecular Biology Recombinases Meiosis Recombinase Amino Acid Sequence Crossing Over Genetic Mitosis Genetics Biochemistry Genetics and Molecular Biology(all) fungi Physical Chromosome Mapping DNA-Binding Proteins Rec A Recombinases enzymes and coenzymes (carbohydrates) Phenotype Multiprotein Complexes Mutation health occupations DMC1 Rad51 Recombinase biological phenomena cell phenomena and immunity Homologous recombination Recombination Protein Binding |
| Zdroj: | Cell. (7):927-940 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2004.10.031 |
| Popis: | Meiotic recombination requires the meiosis-specific RecA homolog Dmc1 as well as the mitotic RecA homolog Rad51. Here, we show that the two meiosis-specific proteins Mei5 and Sae3 are necessary for the assembly of Dmc1, but not for Rad51, on chromosomes including the association of Dmc1 with a recombination hot spot. Mei5, Sae3, and Dmc1 form a ternary and evolutionary conserved complex that requires Rad51 for recruitment to chromosomes. Mei5, Sae3, and Dmc1 are mutually dependent for their chromosome association, and their absence prevents the disassembly of Rad51 filaments. Our results suggest that Mei5 and Sae3 are loading factors for the Dmc1 recombinase and that the Dmc1-Mei5-Sae3 complex is integrated onto Rad51 ensembles and, together with Rad51, plays both catalytic and structural roles in interhomolog recombination during meiosis. |
| Databáze: | OpenAIRE |
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