A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis
| Autor: | Bénédicte Desvoyes, Maria D. Barea, Crisanto Gutierrez, Ainhoa Arana-Echarri |
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| Přispěvatelé: | European Commission, Banco Santander, Fundación Ramón Areces |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mutant Meristem Arabidopsis Plant Science Computational biology 01 natural sciences Plant Roots Fluorescence Cell cycle phase Flow cytometry 03 medical and health sciences Spatio-Temporal Analysis medicine Arabidopsis thaliana Cloning Molecular Cell Size Microscopy Confocal biology medicine.diagnostic_test Cell growth Cell Cycle Robustness (evolution) Cell cycle biology.organism_classification Flow Cytometry Plants Genetically Modified Plant Leaves 030104 developmental biology 010606 plant biology & botany |
| Zdroj: | Nature Plants Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2055-0278 |
| Popis: | Assessing cell proliferation dynamics is crucial to understand the spatiotemporal control of organogenesis. Here we have generated a versatile fluorescent sensor, PlaCCI (plant cell cycle indicator) on the basis of the expression of CDT1a-CFP, H3.1-mCherry and CYCB1;1-YFP, that identifies cell cycle phases in Arabidopsis thaliana. This tool works in a variety of organs, and all markers and the antibiotic resistance are expressed from a single cassette, facilitating the selection in mutant backgrounds. We also show the robustness of PlaCCI line in live-imaging experiments to follow and quantify cell cycle phase progression European Union, and by institutional grants from Banco de Santander and Fundación Ramon Areces to the Centro de Biología Molecular Severo Ochoa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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