Publisher Correction: Identification of cell surface markers and establishment of monolayer differentiation to retinal pigment epithelial cells
| Autor: | Iyadh Douagi, Emma Lardner, Alvaro Plaza Reyes, Fredrik Lanner, Emeline F. Nandrot, Sara Padrell Sánchez, Helder André, Hammurabi Bartuma, Anna Falk, Anders Kvanta, Monica Aronsson, Pankaj Kumar, Sofie Westman, Sandra Petrus-Reurer |
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| Rok vydání: | 2020 |
| Předmět: |
Embryonic stem cells
Science General Physics and Astronomy Retinal Pigment Epithelium Article General Biochemistry Genetics and Molecular Biology Macular Degeneration chemistry.chemical_compound Pigment Monolayer Animals Humans lcsh:Science Neurons Multidisciplinary Cluster of differentiation Cell Differentiation Epithelial Cells Retinal General Chemistry Publisher Correction Embryonic stem cell CD56 Antigen Cell biology Stem-cell research chemistry visual_art visual_art.visual_art_medium Identification (biology) lcsh:Q Laminin Rabbits sense organs Retinal Pigments Biomarkers |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-1 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-17365-4 |
| Popis: | In vitro differentiation of human pluripotent stem cells into functional retinal pigment epithelial (RPE) cells provides a potentially unlimited source for cell based reparative therapy of age-related macular degeneration. Although the inherent pigmentation of the RPE cells have been useful to grossly evaluate differentiation efficiency and allowed manual isolation of pigmented structures, accurate quantification and automated isolation has been challenging. To address this issue, here we perform a comprehensive antibody screening and identify cell surface markers for RPE cells. We show that these markers can be used to isolate RPE cells during in vitro differentiation and to track, quantify and improve differentiation efficiency. Finally, these surface markers aided to develop a robust, direct and scalable monolayer differentiation protocol on human recombinant laminin-111 and −521 without the need for manual isolation. Whilst pigmentation has been used to identify retinal pigment epithelial (RPE) cells, surface markers for these cells remain unclear. Here, the authors define surface markers for the RPE including CD140b, which help produce hPSC-derived RPE cells at a large scale following a robust, direct and scalable monolayer differentiation protocol. |
| Databáze: | OpenAIRE |
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