Perturbed Bone Marrow Monocyte Development Following Burn Injury and Sepsis Promote Hyporesponsive Monocytes
| Autor: | Andrea Szilagyi, Richard L. Gamelli, Kuzhali Muthu, Ravi Shankar, Kurt A. Melstrom, L-K He |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Burn injury medicine.medical_treatment Monocytes Sepsis Mice Bone Marrow medicine Animals Progenitor cell Inflammation Colony-forming unit business.industry Macrophages Monocyte Rehabilitation Dendritic Cells medicine.disease Mice Inbred C57BL Cytokine medicine.anatomical_structure Immunology Emergency Medicine Cytokines Surgery Tumor necrosis factor alpha Bone marrow Burns business |
| Zdroj: | Journal of Burn Care & Research. 29:12-21 |
| ISSN: | 1559-047X |
| DOI: | 10.1097/bcr.0b013e31815fa499 |
| Popis: | The mechanism of monocyte deactivation in critically injured burn patients remains unresolved. Two functionally distinct F4 /80 + Gr-1 + and F4 /80+Gr- 1- monocyte subsets have been characterized based on their homing to inflammatory or noninflammatory tissues, respectively. We hypothesized that the posttraumatic milieu in the bone marrow (BM) blunts the production of "inflammatory" monocytes. C57Blk/J male mice were divided into sham (S), burn (B), and burn sepsis (BS) groups. B and BS received a 15% dorsal scald burn and BS was inoculated with 15K colony forming units Pseudomonas aeruginosa at the burn site. Animals were killed and blood and femoral BM were collected 48, 72, and 96 hours after injury. ER-MP20 + monocyte progenitors were isolated from BM and differentiated into macrophage (MO) or dendritic cells (DCs) and characterized by the cell surface expression of F4 /80 and CDllc, respectively. In both cell types, TLR-4 agonist induced cytokine levels were determined. Results showed a 2-fold increase in the F4 /80 + Gr-1 + subset at 48 hours in BS that started to decline at 72 hours and remained low at 96 hours. ER-MP20 + progenitors isolated at 48 hours exhibited robust MO differentiation potential but a significant decline in the percentage of the F4/80+Gr-1 + subset (P |
| Databáze: | OpenAIRE |
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