Production of Antioxidant Egg White Hydrolysates in a Continuous Stirred Tank Enzyme Reactor Coupled with Membrane Separation Unit
| Autor: | Zorica Knežević-Jugović, Sonja M. Jakovetić, Sanja Grbavčić, Branimir Jugović, Nevena Luković, Jelena R. Jovanović, Milica M. Gvozdenović |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Polyethersulfone ultrafiltration module Antioxidant properties Ultrafiltration Continuous stirred-tank reactor 01 natural sciences Industrial and Manufacturing Engineering Hydrolysate response surface methodology chemistry.chemical_compound 0404 agricultural biotechnology Continuous membrane reactor 010608 biotechnology Response surface methodology Safety Risk Reliability and Quality Chromatography Membrane reactor Chemistry Process Chemistry and Technology Proteolytic enzymes 04 agricultural and veterinary sciences Proteases 040401 food science Egg white protein hydrolysis Papain response surface methodology (RSM) Food Science Egg white |
| Zdroj: | Food and Bioprocess Technology |
| Popis: | The objective of this research was to design an efficient continuously operated membrane reactor with a separation unit for egg white protein (EWP) hydrolysis and production of hydrolysates with improved antioxidant properties. For this purpose, a mechanically stirred tank reactor coupled with the polyethersulfone ultrafiltration module with a molecular weight cutoff of 10 kDa was employed. Several proteolytic enzymes have been tested in order to obtain the best quality of peptide-based formulations intended for human consumption. Among protease from Bacillus licheniformis (Alcalase), protease from Bacillus amyloliquefaciens (Neutrase), and protease from papaya latex (papain), the highest degree of hydrolysis (DH), as well as the best antioxidant properties of obtained hydrolysates, was achieved with Alcalase. The effects of operating variables such as enzyme/substrate ([E]/[S]) ratio, impeller speed, and permeate flow rate were further studied using response surface methodology (RSM) and Box–Behnken experimental design. Results obtained in RSM analysis confirmed that over the studied range [E]/[S] ratio, impeller speed and permeate flow rate had the significant effect on the DH and reactor capacity. The effects of different impeller geometries were also studied and four-bladed propeller stirrer enabled the highest DH. Antioxidant properties were analyzed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH), by the 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging activity, and by the linear voltammetry methods. Results show that the use of Alcalase in the membrane reactor system is of potential interest for the EWP hydrolysis and obtaining value-added egg products. © 2014, Springer Science+Business Media New York. |
| Databáze: | OpenAIRE |
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