Apatite Microtopographies Instruct Signaling Tapestries for Progenitor-Driven New Attachment of Teeth
| Autor: | Xianghong Luan, LeiLei Yin, Thomas G.H. Diekwisch, Giovanni Valdrè, Smit Dangaria, Yoshihiro Ito |
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| Přispěvatelé: | S.J. Dangaria, Y. Ito, L.Yin, G.Valdre, X. Luan, T. Diekwisch |
| Rok vydání: | 2011 |
| Předmět: |
Surface Properties
Biomedical Engineering Bioengineering Biochemistry Green fluorescent protein Biomaterials Extracellular matrix Dental Materials Mice stomatognathic system Cell Adhesion Extracellular Animals Periodontal fiber Progenitor cell Cell adhesion Dental alveolus Progenitor Dental Implants Chemistry APATITE 3T3 Cells Original Articles TISSUE ENGINEERING Cell biology stomatognathic diseases Durapatite Guided Tissue Regeneration Periodontal Porosity Tooth STEM CELLS Stem Cell Transplantation Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 17:279-290 |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Dimension and structure of extracellular matrix surfaces have powerful influences on cell shape, adhesion, and gene expression. Here we show that natural tooth root topographies induce integrin-mediated extracellular matrix signaling cascades in tandem with cell elongation and polarization to generate physiological periodontium-like tissues. In this study we replanted surface topography instructed periodontal progenitors into rat alveolar bone sockets for 8 and 16 weeks, resulting in complete reattachment of tooth roots to the surrounding alveolar bone with a periodontal fiber apparatus closely matching physiological controls along the entire root surface. Displacement studies and biochemical analyses confirmed that progenitor-based engineered periodontal tissues were similar to control teeth and uniquely derived from preimplantation green fluorescent protein (GFP)-labeled progenitors. Together, these studies illustrate the capacity of natural extracellular surface topographies to instruct progenitor cell populations to fully regenerate complex cellular and structural morphologies of tissues once lost to disease. We suggest that our strategy could be used for the replantation of teeth lost due to trauma or as a novel approach for tooth replacement using tooth-shaped replicas. |
| Databáze: | OpenAIRE |
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