Study of the initial phase of biofilm formation using a biofomic approach
| Autor: | Jean-Paul Dehaye, Carole Nagant, Michel Devleeschouwer, Marie Tré-Hardy |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Bacteriological Techniques biology Strain (chemistry) Rhamnose Biofilm Swarming motility biochemical phenomena metabolism and nutrition biology.organism_classification Microbiology Staining chemistry.chemical_compound chemistry Biofilms Pseudomonas aeruginosa Crystal violet Molecular Biology Bacteria Pseudomonadaceae |
| Zdroj: | Journal of Microbiological Methods. 82:243-248 |
| ISSN: | 0167-7012 |
| Popis: | article i nfo Article history: Aims: The purpose of this work was to study the initial steps of formation of a biofilm using the BioFilm Ring Test ® and the Crystal violet staining technique. Methods and results: Eight strains of Pseudomonas aeruginosa were studied. The two methods revealed that four strains formed a rapid biofilm. The biofilm formed by these strains was detected after only 45 min with the BioFilm Ring Test ® and after 6 h with the Crystal violet method. The enumeration of bacteria of the PA01 strain confirmed that, after 30 min, a significant amount of bacteria had attached on the bottom of the culture wells. After 48 h the Crystal violet method detected a biofilm with all strains. The four strains which rapidly formed a biofilm did not differ from the slow-forming strains by their mucoid character or their swarming motility or their synthesis of rhamnose. They showed higher swimming mobility. Conclusions: Our results show that the BioFilm Ring Test ® is a method specially suited for the study of the initial phase of the formation of a biofilm. Significance and impact of study: The BioFilm Ring Test ® is an easy and rapid alternative to the Crystal violet staining and the enumeration methods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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