Gastric histidine decarboxylase
| Autor: | Phyllis E. Powell, M. Ashwini Kumar |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Male
Food intake Carboxy-Lyases Biophysics Biochemistry Methods Gastric mucosa medicine Animals Histidine Radiometry Molecular Biology Antrum chemistry.chemical_classification Carbon Isotopes Aromatic L-amino acid decarboxylase biology Cell Biology Hydrogen-Ion Concentration Histidine decarboxylase Enzyme assay Rats Kinetics Enzyme medicine.anatomical_structure chemistry Gastric Mucosa Dopa Decarboxylase biology.protein Histidine decarboxylase activity |
| Zdroj: | Analytical Biochemistry. 22:485-492 |
| ISSN: | 0003-2697 |
| Popis: | A simple method is described for determining histidine decarboxylase activity. It depends on the measurement of 14 CO 2 liberated from tracer carboxyl- 14 C- l -histidine. The method is sensitive enough to permit measurements in small samples of gastric mucosa of individual rats. It is specific in that dopa decarboxylase does not interfere. The enzyme has constant K m values of 5 × 10 −4 M between pH 6 and 8, while the maximum reaction velocity has an optimum near pH 7. The enzyme content is high in the ruminal and main gastric and low in pyloric antral mucosa. Food intake enhances enzyme activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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