Nix is a male-determining factor in the Asian tiger mosquito Aedes albopictus
| Autor: | Xiaocong Li, Xiao-Guang Chen, Yijie Zhao, Jinbao Gu, Binbin Jin, Peiwen Liu, Zhijian Jake Tu, James K. Biedler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Gene isoform Male Aedes albopictus Aedes aegypti 01 natural sciences Biochemistry Article 03 medical and health sciences Transcription (biology) Aedes Animals Molecular Biology Gene Gene knockout 030304 developmental biology Genetics 0303 health sciences biology Base Sequence fungi virus diseases Sex Determination Processes biology.organism_classification 010602 entomology Insect Science RNA splicing Tiger mosquito Insect Proteins Female Sequence Alignment |
| Zdroj: | Insect Biochem Mol Biol |
| Popis: | The initial signal that governs sex determination is highly variable among insects. A homolog of Nix, the male-determining factor in Aedes aegypti, was previously found in the Asian tiger mosquito Ae. albopictus. Here we show that the Ae. albopictus Nix (AalNix) is more complex in gene structure and splice isoforms than its Ae. aegypti homolog (AaeNix). AalNix shows a similar transcription profile compared to AaeNix. CRISPR/Cas9-mediated knockouts of AalNix in vivo and in the Ae. albopictus C6/36 cells lead to a shift of dsx and fru splicing towards the female isoforms. G0 knockout males showed feminization and deformities including feminized antennae, absence or partial absence of gonocoxites, gonostyli, testes and accessory glands, and the formation of ovaries. Despite ~70 MY of divergence, Nix functions as a conserved male-determining factor in the two most important arboviral vectors, namely Ae. aegypti and Ae. albopictus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|