TREM-1 and TREM-2 Expression on CD14+ Cells in Bronchoalveolar Lavage Fluid in Pulmonary Sarcoidosis and Hypersensitivity Pneumonitis in the Context of T Cell Immune Response

Autor: J Urban, Brian König, M Suchankova, Maria Bucova, Ivan Majer, E Tibenska, K Szaboova, Frantisek Sandor, Eva Tedlova, M Bobovcak, I Jonner, M Ganovska
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Adult
Male
Article Subject
Receptor expression
T cell
T-Lymphocytes
Immunology
Lipopolysaccharide Receptors
Enzyme-Linked Immunosorbent Assay
Monocytes
03 medical and health sciences
0302 clinical medicine
Immune system
Sarcoidosis
Pulmonary
medicine
Pathology
Cytotoxic T cell
Humans
RB1-214
Receptors
Immunologic
Aged
Proportional Hazards Models
Inflammation
Membrane Glycoproteins
medicine.diagnostic_test
business.industry
Brain Neoplasms
Interleukin-6
Cell Biology
Glioma
Middle Aged
medicine.disease
Triggering Receptor Expressed on Myeloid Cells-1
Interleukin-10
030104 developmental biology
Bronchoalveolar lavage
medicine.anatomical_structure
Immune System
Female
Sarcoidosis
business
Bronchoalveolar Lavage Fluid
CD8
Hypersensitivity pneumonitis
030215 immunology
Research Article
Zdroj: Mediators of Inflammation, Vol 2020 (2020)
Mediators of Inflammation
ISSN: 1466-1861
0962-9351
Popis: Background. Sarcoidosis and hypersensitivity pneumonitis (HP) are immunologically mediated processes caused by hypersensitivity reaction accompanied by similar features including lymphocytic alveolitis and granuloma formation. Recent studies describe the role of TREM receptors in T cell activation, differentiation, and granuloma formation. Alveolar macrophages activation via TREM receptors may be the key factor mediating subsequent immune response. The aim of the study was to analyse TREM-1 and TREM-2 expression to identify further molecular mechanisms participating in the immunopathogenesis of sarcoidosis and HP. Methods. Flow cytometry was performed to analyse TREM-1 and TREM-2 expression on CD14+ cells in bronchoalveolar lavage fluid from patients having sarcoidosis or HP and a control group. Results. The study proved increased TREM-1 expression on alveolar macrophages in pulmonary sarcoidosis and diminished TREM-1 expression in HP-Sarcoidosis: median: 76.7; HP: median: 29.9; control: median: 53.3, (sarcoidosis versus HP: p<0.001; sarcoidosis versus control: p<0.05). TREM-2 expression was increased in both, sarcoidosis and HP-sarcoidosis: median: 34.79; HP: median: 36.00; control: median: 12.98, (sarcoidosis versus control: p<0.05; HP versus control: p<0.05). Correlation analysis showed negative correlation between TREM-1 and total number of CD8+ cytotoxic T cells. In sarcoidosis TREM-1 expression decreased with changes of HRCT image, decrease in CD4/CD8 ratio and decrease in DLCO. Conclusions. Differences in TREM receptor expression in sarcoidosis (increase in TREM-1 and TREM-2) and HP (increase in TREM-2) and correlation analysis suggests that activation via TREM may participate in typical immunological characteristics of sarcoidosis and HP.
Databáze: OpenAIRE
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