Molecular differentiation of Mycoplasma gallisepticum and Mycoplasma imitans strains by pulsed-field gel electrophoresis and random amplified polymorphic DNA
| Autor: | Isabelle Kempf, C. Marois, F. Dufour-Gesbert |
|---|---|
| Přispěvatelé: | Kempf, Isabelle, Laboratoire d'études et de recherches avicoles, porcines et piscicoles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Mycoplasma gallisepticum
Turkeys MESH: Poultry Diseases Microbiology MESH: Random Amplified Polymorphic DNA Technique chemistry.chemical_compound MESH: Mycoplasma Mycoplasma Genetic variation parasitic diseases Pulsed-field gel electrophoresis Animals Mycoplasma Infections MESH: Animals MESH: Genetic Variation Poultry Diseases Genetics Gel electrophoresis MESH: Turkeys biology Strain (biology) MESH: Chickens Genetic Variation Reproducibility of Results food and beverages General Medicine biochemical phenomena metabolism and nutrition MESH: Mycoplasma Infections biology.organism_classification bacterial infections and mycoses [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Electrophoresis Gel Pulsed-Field Random Amplified Polymorphic DNA Technique RAPD MESH: Reproducibility of Results MESH: Electrophoresis Gel Pulsed-Field chemistry Mycoplasma imitans [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Chickens DNA geographic locations |
| Zdroj: | Journal of Veterinary Medicine Series B Journal of Veterinary Medicine Series B, 2001, 48 (9), pp.695-703 |
| Popis: | Pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) analysis were used to compare 21 Mycoplasma gallisepticum strains and five M. imitans strains. Each strain of M. gallisepticum typed by PFGE and RAPD methods was genetically quite unique and RAPD and PFGE fingerprinting enabled strain characterization. Relationships between the M. gallisepticum and M. imitans strains were established and dendrograms were drawn from PFGE and RAPD patterns. PFGE group A and RAPD group D were significantly associated with M. imitans strains (P < 0.05). Three M. imitans strains shared the same PFGE and RAPD patterns. The two M. gallisepticum vaccine strains had singular PFGE and RAPD patterns. Thus, PFGE and RAPD can be used to investigate disease outbreaks in vaccinated flocks or for epidemiological tracking. For M. gallisepticum, the RAPD and PFGE discriminatory powers were superior to 0.95 and the in vitro, in ovo and in vivo reproducibility of RAPD and PFGE was 100%. The RAPD drawback was the inconsistent band intensity complicating the interpretation of patterns, while the PFGE limit was its low typeability (86%). Thus, these two molecular typing methods seemed complementary for M. gallisepticum epidemiological studies. |
| Databáze: | OpenAIRE |
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