Antibacterial Properties of Mussel-Inspired Polydopamine Coatings Prepared by a Simple Two-Step Shaking-Assisted Method
| Autor: | Rattapol Pinnaratip, Yuesheng Gao, Lei Pan, Mikhail Trought, Bruce P. Lee, Kathryn A. Perrine, Pegah Kord Forooshani, Elizabeth Polega, Chito E. Kendrick, Md. Saleh Akram Bhuiyan, Kevin Thomson |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
health care facilities
manpower and services education Nanoparticle hydrogen peroxide 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences lcsh:Chemistry chemistry.chemical_compound Coating surgical mesh Hydrogen peroxide polydopamine Original Research Polypropylene Catechol coating General Chemistry catechol 021001 nanoscience & nanotechnology Phosphate 0104 chemical sciences Chemistry antibacterial lcsh:QD1-999 chemistry engineering Cyclic voltammetry 0210 nano-technology Layer (electronics) Nuclear chemistry |
| Zdroj: | Frontiers in Chemistry Frontiers in Chemistry, Vol 7 (2019) |
| ISSN: | 2296-2646 |
| Popis: | A simple two-step, shaking-assisted polydopamine (PDA) coating technique was used to impart polypropylene (PP) mesh with antimicrobial properties. In this modified method, a relatively large concentration of dopamine (20 mg ml−1) was first used to create a stable PDA primer layer, while the second step utilized a significantly lower concentration of dopamine (2 mg ml−1) to promote the formation and deposition of large aggregates of PDA nanoparticles. Gentle shaking (70 rpm) was employed to increase the deposition of PDA nanoparticle aggregates and the formation of a thicker PDA coating with nano-scaled surface roughness (RMS = 110 nm and Ra = 82 nm). Cyclic voltammetry experiment confirmed that the PDA coating remained redox active, despite extensive oxidative cross-linking. When the PDA-coated mesh was hydrated in phosphate saline buffer (pH 7.4), it was activated to generate 200 μM hydrogen peroxide (H2O2) for over 48 h. The sustained release of low doses of H2O2 was antibacterial against both gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. PDA coating achieved 100% reduction (LRV ~3.15) when incubated against E. coli and 98.9% reduction (LRV ~1.97) against S. epi in 24 h. |
| Databáze: | OpenAIRE |
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