The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission
| Autor: | Julio Chavez-Galarza, Christian Tellgren-Roth, Joachim R. de Miranda, M. Alice Pinto, Peter J. Neumann, Orlando Yañez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Varroidae 030106 microbiology lcsh:Medicine Tecnología de modificación genética Viral quasispecies Virus-host interactions Zoologi Article Virus Ácaro law.invention Evolutionsbiologi Composición Genética 03 medical and health sciences Negative selection law Deformed wing virus Mite Animals RNA Viruses lcsh:Science Viral evolution Evolutionary Biology Multidisciplinary Ecology biology Abeja Occidental (Apis Mellifera) lcsh:R Pupa Bees biology.organism_classification Virology Quasispecies Microbiology (Microbiology in the medical area to be 30109) 030104 developmental biology Transmission (mechanics) Varroa destructor 570 Life sciences lcsh:Q Arachnid Vectors Adaptation Zoology |
| Zdroj: | Scientific Reports Instituto Nacional de Innovación Agraria INIA-Institucional instacron:INIA Repositorio Institucional-INIA Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Yañez, Orlando; Chávez-Galarza, Julio; Tellgren-Roth, Christian; Pinto, Alice; Neumann, Peter; de Miranda, Joachim (2020). The honeybee (Apis mellifera) developmental state shapes the genetic composition of the deformed wing virus-A quasispecies during serial transmission. Scientific reports, 10(1), p. 5956. Springer Nature 10.1038/s41598-020-62673-w Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| DOI: | 10.7892/boris.144377 |
| Popis: | The main biological threat to the western honeybee (Apis mellifera) is the parasitic mite Varroa destructor, largely because it vectors lethal epidemics of honeybee viruses that, in the absence of this mite, are relatively innocuous. The severe pathology is a direct consequence of excessive virus titres caused by this novel transmission route. However, little is known about how the virus adapts genetically during transmission and whether this influences the pathology. Here, we show that upon injection into honeybee pupae, the deformed wing virus type-A (DWV-A) quasispecies undergoes a rapid, extensive expansion of its sequence space, followed by strong negative selection towards a uniform, common shape by the time the pupae have completed their development, with no difference between symptomatic and asymptomatic adults in either DWV titre or genetic composition. This suggests that the physiological and molecular environment during pupal development has a strong, conservative influence on shaping the DWV-A quasispecies in emerging adults. There was furthermore no evidence of any progressive adaptation of the DWV-A quasispecies to serial intra-abdominal injection, simulating mite transmission, despite the generation of ample variation immediately following each transmission, suggesting that the virus either had already adapted to transmission by injection, or was unaffected by it. Many thanks to the Uppsala Genome Centre for sequencing services, to Kaspar Roth at the Institute of Bee Health (IBH) for technical support, to Pilar de la Rua for analytical advice and to the four excellent anonymous reviewers for constructive comments on the manuscript. Bioinformatic analyses were performed at the Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX). This project was funded by grant C09.0048 from the EU-COST FA0803 action, grant SNF 31003A_141006/1 from the Swiss National Science Foundation and the Vinetum Foundation. Open access funding provided by Swedish University of Agricultural Sciences. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|