iTRAQ-Based Quantitative Proteomic Analysis of Heat Stress-Induced Mechanisms in Pepper Seedlings
| Autor: | Xuexiao Zou, Chen Wenchao, Li Xuefeng, Fangjun Tan, Chunhai Jiao, Xilu Zhang, Yang Sha, Jing Wang, Xiongze Dai, Jingshuang Song, Liang Chengliang, Fei Wang, Niran Juntawong |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Proteomics Bioinformatics Physiological Plant Science Carbohydrate metabolism Biology Photosynthesis 01 natural sciences General Biochemistry Genetics and Molecular Biology Heat stress 03 medical and health sciences Pepper Food science Agricultural Science Molecular Biology 030304 developmental biology 0303 health sciences General Neuroscience food and beverages General Medicine Phenotype Metabolic pathway Proteome Medicine Signal transduction General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | PeerJ PeerJ, Vol 9, p e11509 (2021) |
| DOI: | 10.21203/rs.3.rs-46970/v1 |
| Popis: | Background As one of the most important vegetable crops, pepper has rich nutritional value and high economic value. Increasing heat stress due to the global warming has a negative impact on the growth and yield of pepper. Methods To understand the heat stress response mechanism of pepper, an iTRAQ-based quantitative proteomic analysis was employed to identify possible heat-responsive proteins and metabolic pathways in 17CL30 and 05S180 pepper seedlings under heat stress. Result In the present study, we investigated the changes of phenotype, physiology, and proteome in heat-tolerant (17CL30) and heat-sensitive (05S180) pepper cultivars in response to heat stress. Phenotypic and physiological changes showed that 17CL30 had a stronger ability to resist heat stress compared with 05S180. In proteomic analysis, a total of 3,874 proteins were identified, and 1,591 proteins were considered to participate in the process of heat stress response. According to bioinformatic analysis of heat-responsive proteins, the heat tolerance of 17CL30 might be related to a higher ROS scavenging, photosynthesis, signal transduction, carbohydrate metabolism, and stress defense, compared with 05S180. |
| Databáze: | OpenAIRE |
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